Abstract：
Skin aging has become a major urgent problem to be solved. Evidence reveals that oxidation and glycosylation are two dominant inducements of aging. Resveratrol (RES) with outstanding anti-oxidant effect and carnosine (CAR) with superb anti-glycation property were selected as two model drugs to evaluate the feasibility of their synergistic anti-aging effect. RES and CAR at the most desired mass ratio, supplying the most superior synergistic anti-aging effects were further encapsulated in liposomes (LP), which were separately coated with chitosan (CS) and catechol chitosan (Cat-CS) to increase the transdermal penetration. Their anti-aging efficacy was explored in human skin fibroblast (HSF) and human immortalized keratinocytes (HaCaT) cells, as well as the back skin of guinea pigs. Herein, RES and CAR at the mass ratio of 2:1 exhibited the most ideal synergistic anti-aging effect. The constructed liposomes have been shown to possess excellent fundamental properties and sustained-release properties. The aging-related indicator levels in the two cells and guinea pigs were obviously improved for the RES + CAR@Cat-CS-LP group. Additionally, skin appearance, tissue morphology, and collagen content were visibly improved, indicating its perfect anti-aging effect. In conclusion, RES + CAR@Cat-CS-LP is expected to be exploited as a potential anti-aging drug delivery system.
摘要：
皮肤老化已成为亟待解决的重大问题。证据表明，氧化和糖基化是衰老的两个主要诱因。选择抗氧化作用突出的白藜芦醇(RES)和抗糖基化作用优异的肌肽(CAR)作为两种模型药物,评价其协同抗衰老作用的可行性。RES和CAR在最理想的质量比，提供最优越的协同抗衰老作用进一步封装在脂质体（LP），分别用壳聚糖（CS）和儿茶酚壳聚糖（Cat-CS）包被以增加经皮渗透。在人皮肤成纤维细胞（HSF）和人永生化角质形成细胞（HaCaT）细胞中探索了它们的抗衰老功效，以及豚鼠的背部皮肤。在这里，RES和CAR在质量比为2:1时表现出最理想的协同抗衰老效果。已显示所构建的脂质体具有优异的基本性质和持续释放性质。对于RES+CAR@Cat-CS-LP组,两种细胞和豚鼠中的衰老相关指标水平显著提高。此外,皮肤外观,组织形态学,胶原蛋白含量明显改善，说明其完美的抗衰老效果。总之,RES+CAR@Cat-CS-LP有望被用作潜在的抗衰老药物递送系统。
