PDF(Pubmed)
Abstract：
Perfused placentas provide an excellent and accessible model for microvascular dissection, microsuturing and microanastomosis training - particularly in the early microsurgical learning curve. This way, a significant amount of live animals can be spared.
We present the Zurich Microsurgery Lab protocol, detailing steps for obtaining, selecting, cleaning, flushing, cannulating, and preserving human placentas - as well as microsurgical training examples - in a tried-and-true, safe, cost-effective, and high-yield fashion.
Our technique enables highly realistic microsurgical training (microdissection, microvascular repair, microanastomosis) based on readily available materials. Proper handling, preparation, and preservation of the perfused placenta models is key.
We present the Zurich Microsurgery Lab protocol, detailing steps for obtaining, selecting, cleaning, flushing, cannulating, and preserving human placentas - as well as microsurgical training examples - in a tried-and-true, safe, cost-effective, and high-yield fashion.
Our technique enables highly realistic microsurgical training (microdissection, microvascular repair, microanastomosis) based on readily available materials. Proper handling, preparation, and preservation of the perfused placenta models is key.
摘要：
背景：灌注胎盘为微血管夹层提供了一个出色且可访问的模型，微缝合和微吻合训练-特别是在早期显微外科学习曲线。这边,大量的活体动物可以幸免。
方法：我们提出了苏黎世显微外科实验室协议，详细说明获取步骤，选择,清洁,冲洗,插管,和保存人类胎盘-以及显微外科训练的例子-在一个久经考验的和真实的，安全,成本效益高,和高产量的时尚。
结论：我们的技术可以进行高度逼真的显微外科训练（显微解剖，微血管修复,微吻合）基于现成的材料。处理得当,准备,保存灌注的胎盘模型是关键。
方法：我们提出了苏黎世显微外科实验室协议，详细说明获取步骤，选择,清洁,冲洗,插管,和保存人类胎盘-以及显微外科训练的例子-在一个久经考验的和真实的，安全,成本效益高,和高产量的时尚。
结论：我们的技术可以进行高度逼真的显微外科训练（显微解剖，微血管修复,微吻合）基于现成的材料。处理得当,准备,保存灌注的胎盘模型是关键。
