PDF(Pubmed)
Abstract：
Endozoicomonas are often predominant bacteria and prominently important in coral health. Their role in dimethylsulfoniopropionate (DMSP) degradation has been a subject of discussion for over a decade. A previous study found that Endozoicomonas degraded DMSP through the dddD pathway. This process releases dimethyl sulfide, which is vital for corals coping with thermal stress. However, little is known about the related gene regulation and metabolic abilities of DMSP metabolism in Endozoicomonadaceae. In this study, we isolated a novel Endozoicomonas DMSP degrader and observed a distinct DMSP metabolic trend in two phylogenetically close dddD-harboring Endozoicomonas species, confirmed genetically by comparative transcriptomic profiling and visualization of the change of DMSP stable isotopes in bacterial cells using nanoscale secondary ion spectrometry. Furthermore, we found that DMSP cleavage enzymes are ubiquitous in coral Endozoicomonas with a preference for having DddD lyase. We speculate that harboring DMSP degrading genes enables Endozoicomonas to successfully colonize various coral species across the globe.
摘要：
内生单胞菌通常是主要细菌，在珊瑚健康中非常重要。它们在二甲基磺基丙酸酯（DMSP）降解中的作用已成为十多年来讨论的主题。先前的研究发现，内生单胞菌通过dddD途径降解DMSP。这个过程释放二甲基硫醚，这对珊瑚应对热应力至关重要。然而,关于内生子科DMSP代谢的相关基因调控和代谢能力知之甚少。在这项研究中,我们分离了一种新型的内孢子单胞菌DMSP降解剂，并在两个系统发育上接近dddD的内孢子单胞菌物种中观察到了明显的DMSP代谢趋势，通过比较转录组学分析和使用纳米级二次离子光谱法对细菌细胞中DMSP稳定同位素的变化进行可视化来确认遗传。此外，我们发现，DMSP裂解酶在珊瑚Entozoicomonas中普遍存在，并且偏爱DddD裂解酶。我们推测，携带DMSP降解基因使Entozoicomonas能够成功地在全球范围内定居各种珊瑚物种。
