{Reference Type}: Journal Article
{Title}: Similar but different: Characterization of dddD gene-mediated DMSP metabolism among coral-associated Endozoicomonas.
{Author}: Chiou YJ;Chan YF;Yu SP;Lu CY;Hsiao SS;Chiang PW;Hsu TC;Liu PY;Wada N;Lee Y;Jane WN;Lee DC;Huang YW;Tang SL;
{Journal}: Sci Adv
{Volume}: 9
{Issue}: 47
{Year}: 2023 11 24
{Factor}: 14.957
{DOI}: 10.1126/sciadv.adk1910
{Abstract}: Endozoicomonas are often predominant bacteria and prominently important in coral health. Their role in dimethylsulfoniopropionate (DMSP) degradation has been a subject of discussion for over a decade. A previous study found that Endozoicomonas degraded DMSP through the dddD pathway. This process releases dimethyl sulfide, which is vital for corals coping with thermal stress. However, little is known about the related gene regulation and metabolic abilities of DMSP metabolism in Endozoicomonadaceae. In this study, we isolated a novel Endozoicomonas DMSP degrader and observed a distinct DMSP metabolic trend in two phylogenetically close dddD-harboring Endozoicomonas species, confirmed genetically by comparative transcriptomic profiling and visualization of the change of DMSP stable isotopes in bacterial cells using nanoscale secondary ion spectrometry. Furthermore, we found that DMSP cleavage enzymes are ubiquitous in coral Endozoicomonas with a preference for having DddD lyase. We speculate that harboring DMSP degrading genes enables Endozoicomonas to successfully colonize various coral species across the globe.