PDF(Pubmed)
Abstract：
This study aimed to investigate the anti-inflammatory and wound healing effects of the polysaccharide extract from Opuntia ficus-indica cladodes (TPL-Ofi) using a rat cutaneous wound model. After anaesthesia, four 7-mm-diameter dorsal wounds per animal (n = 6/group for each experimental day of evaluation) were created in female Wistar rats using a surgical punch. The animals were treated topically twice daily with TPL-Ofi (0.01-1%; treated group) or sterile saline (control group) for a period of 21 days. Ulcerated tissue was collected for analysis of histological parameters (inflammation score, number of polymorphonuclear, mononuclear, fibroblast/myofibroblasts and blood vessels), immunohistochemical (fibroblast growth factor 2 [FGF-2]) and oxidative stress markers (myeloperoxidase [MPO] and glutathione [GSH]). After 21 days of treatment, body weight, net organ weight and plasma biochemical levels were measured. TPL-Ofi, containing a total carbohydrate content of 65.5% and uronic acid at 2.8%, reduced oedema on the second day and increased the nociceptive threshold on the second and third days. TPL-Ofi reduced mononuclear infiltrate on the second and MPO activity on the fifth day. TPL-Ofi increased GSH levels on the second day, as well as fibroblast/myofibroblasts counts, neoangiogenesis and FGF-2 levels on the fifth and seventh days. No changes were observed in body weight, net organ weight or toxicology assessment. Topical application of TPL-Ofi exhibited anti-inflammatory and antinociceptive effects, ultimately improving wound healing in cutaneous wounds.
摘要：
本研究旨在使用大鼠皮肤伤口模型研究仙人掌多糖提取物（TPL-Ofi）的抗炎和伤口愈合作用。麻醉后,使用手术打孔器在雌性Wistar大鼠中创建每只动物四个直径7毫米的背侧伤口（每个实验日的评估时间为n=6/组）。每天用TPL-Ofi(0.01-1%;治疗组)或无菌盐水(对照组)局部治疗动物两次,持续21天。收集溃疡组织进行组织学参数分析（炎症评分，多形核的数量，单核,成纤维细胞/肌成纤维细胞和血管），免疫组织化学（成纤维细胞生长因子2[FGF-2]）和氧化应激标记（髓过氧化物酶[MPO]和谷胱甘肽[GSH]）。治疗21天后，体重,测量净器官重量和血浆生化水平。TPL-Ofi,含有65.5%的总碳水化合物含量和2.8%的糖醛酸，第二天水肿减少，第二天和第三天伤害性阈值增加。TPL-Ofi在第2天减少了单核浸润，在第5天减少了MPO活性。TPL-Ofi在第二天增加了GSH水平，以及成纤维细胞/肌成纤维细胞计数，第五天和第七天的新血管生成和FGF-2水平。体重没有变化，净器官重量或毒理学评估。TPL-Ofi的局部应用表现出抗炎和镇痛作用，最终改善皮肤伤口的伤口愈合。
