Abstract：
Collagen peptides play an important role in the increasing use of collagen peptides as dietary supplements in food and beverages and as bioactive ingredients in cosmetics, healthcare, and pharmaceuticals. Collagenase enzymatically cleaves gelatin to produce collagen polypeptides. However, the enzymatic activity of collagenase is very low (25900 U) and does not allow for adequate enzymatic digestion. Therefore, proteases are used to assist in enzymatic digestion. Porcine gelatin, bovine gelatin, and fish protein gum were enzymatically digested, and the content of collagen peptides in the enzymatically digested lyophilized powder was identified by high-performance liquid chromatography and mass spectrometry, and then the content of the desired collagen peptides was increased by isolation and purification, and the result of the determination was that the content of collagen peptides was the highest after enzymatic digestion and isolation and purification with the use of porcine gelatin as the raw material, and the content of the collagen peptides was about 45.47%. β-nicotinamide mononucleotide (NMN) was mixed with the prepared samples to determine its antioxidant properties and ability to promote the growth of human dermal fibroblasts. The results showed that the antioxidant capacity was enhanced with the increase of collagen polypeptide content, and NMN could promote the scavenging of DPPH• and •OH free radicals by collagen polypeptides. The ability to promote the growth of human dermal fibroblasts was enhanced with the increase of collagen polypeptide content. This paper aimed to prepare a high content of collagen polypeptides from three raw materials, porcine gelatin, bovine gelatin, and fish protein gum, and further to determine the biological activities.
摘要：
胶原蛋白肽在增加胶原蛋白肽作为食品和饮料中的膳食补充剂以及作为化妆品中的生物活性成分的用途中起着重要作用。healthcare,和药物。胶原酶酶促裂解明胶以产生胶原多肽。然而,胶原酶的酶活性非常低（25900U），不允许足够的酶消化。因此,蛋白酶用于辅助酶消化。猪明胶,牛明胶,鱼胶蛋白被酶消化，酶消化冻干粉中胶原肽的含量通过高效液相色谱和质谱,然后通过分离和纯化增加所需胶原肽的含量，以猪明胶为原料经酶法消化、分离纯化后胶原肽含量最高,胶原肽的含量约为45.47%。将β-烟酰胺单核苷酸（NMN）与制备的样品混合，以确定其抗氧化特性和促进人真皮成纤维细胞生长的能力。结果表明,随着胶原多肽含量的增加,抗氧化能力增强,和NMN可以促进胶原蛋白多肽清除DPPH·和·OH自由基。随着胶原蛋白多肽含量的增加，促进人真皮成纤维细胞生长的能力增强。本文以三种原料为原料制备了一种高含量的胶原多肽,猪明胶,牛明胶,和鱼蛋白胶，并进一步确定其生物活性。
