PDF(Pubmed)
Abstract：
OBJECTIVE: With the circulation of high pathogenicity avian influenza viruses having intensified considerably in recent years, the European Union is considering the vaccination of farmed birds. A prerequisite for this vaccination is the implementation of drastic surveillance protocols. Environmental sampling is a relevant alternative to animal sampling. However, environmental samples often contain inhibitory compounds in large enough quantities to inhibit RT-qPCR reactions. As bovine serum albumin is a molecule used in many fields to overcome this inhibitory effect, we tested its use on dust samples from poultry farms in areas heavily affected by HPAIV epizootics. Our results show that its use significantly increases the sensitivity of the method.
摘要：
目的:随着近年来高致病性禽流感病毒传播的加剧,欧盟正在考虑对养殖鸟类进行疫苗接种。这种疫苗接种的先决条件是实施严格的监测方案。环境采样是动物采样的相关替代方法。然而,环境样品通常含有足够大量的抑制性化合物以抑制RT-qPCR反应。由于牛血清白蛋白是许多领域用来克服这种抑制作用的分子，我们在受HPAIV流行病严重影响的地区的家禽养殖场的灰尘样本中测试了其使用情况。我们的结果表明，它的使用显着提高了方法的灵敏度。
