Abstract：
Wet meadows, a type of wetland, are vulnerable to climate change and human activity, impacting soil properties and microorganisms that are crucial to the ecosystem processes of wet meadows. To decipher the ecological mechanisms and processes involved in wet meadows, it is necessary to examine the bacterial communities associated with plant roots. To gain valuable insight into the microbial dynamics of alpine wet meadows, we used Illumina MiSeq sequencing to investigate how environmental factors shape the bacterial communities thriving in the rhizosphere and rhizoplane of three plant species: Cremanthodium ellisii, Caltha scaposa, and Cremanthodium lineare. The most abundant bacterial phyla in rhizosphere and rhizoplane were Proteobacteria > Firmicutes > Actinobacteria, while Macrococcus, Lactococcus, and Exiguobacterium were the most abundant bacterial genera between rhizosphere and rhizoplane. The mantel test, network, and structure equation models revealed that bacterial communities of rhizosphere were shaped by total nitrogen (TN), soil water content (SWC), soil organic carbon (SOC), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), pH, however, rhizoplane bacterial communities exhibited varying results. The bacterial communities exhibited significant heterogeneity, with stochastic process predominating in both the rhizosphere and rhizoplane. PICRUSt2 and FAPROTAX analysis revealed substantial differences in key biogeochemical cycles and metabolic functional predictions. It was concluded that root compartments significantly influenced the bacterial communities, although plant species and elevation asserted varying effects. This study portrays how physicochemical properties, plant species, and elevations can shift the overall structure and functional repertoire of bacterial communities in alpine wet meadows.
摘要：
潮湿的草地，一种湿地，容易受到气候变化和人类活动的影响，影响土壤性质和微生物，这对湿草甸的生态系统过程至关重要。为了破译湿草甸的生态机制和过程，有必要检查与植物根部相关的细菌群落。为了获得对高山湿草甸微生物动力学的宝贵见解，我们使用IlluminaMiSeq测序来研究环境因素如何塑造三种植物物种的根际和根际平面中蓬勃发展的细菌群落：Calthascaposa,和Cremanthodiumlineare。根际和根际平面中最丰富的细菌门是变形菌>厚壁菌>放线菌，而大型球菌，乳球菌,外生细菌是根际和根际平面之间最丰富的细菌属。壁炉架测试，网络,和结构方程模型表明，根际细菌群落由总氮(TN)形成，土壤含水量（SWC），土壤有机碳(SOC)，微生物生物量碳(MBC)，微生物生物量氮(MBN)，pH值,然而,根际平面细菌群落表现出不同的结果。细菌群落表现出显著的异质性，随机过程在根际和根际平面中占主导地位。PICRUSt2和FAPROTAX分析揭示了关键生物地球化学循环和代谢功能预测的实质性差异。结论是，根区室显着影响细菌群落，尽管植物种类和海拔具有不同的影响。这项研究描述了物理化学性质，植物物种,和海拔可以改变高山湿草甸细菌群落的整体结构和功能库。
