Abstract：
The retina is a high-metabolism tissue composed of various cell types with complex functions that relies heavily on the blood supply to maintain homeostasis. Retinal ischemia-reperfusion injury is a critical pathogenic mechanism in glaucoma, and changes in lipid molecules may lead to retinal tissue damage. However, retinal lipid profile alterations caused by this mechanism remain unclear. Thus, this study employed a retinal ischemia-reperfusion model to analyze changes in the lipid profile between sham-operated and ischemia-reperfusion groups. We discovered that ischemia-reperfusion injury-induced alterations in 338 lipid molecules, which potentially caused lipid droplet formation and mitochondrial damage. Notably, we identified characteristic changes in various lipids, including cholesterol esters, cardiolipin, and ceramide, which may serve as potential biomarkers for assessing the severity of retinal injury and therapeutic interventions. The ischemia-reperfusion-specific features identified in this study provide a more comprehensive understanding of the pathophysiological mechanisms underlying this condition.
摘要：
视网膜是由具有复杂功能的各种细胞类型组成的高代谢组织,其在很大程度上依赖于血液供应来维持体内平衡。视网膜缺血再灌注损伤是青光眼的重要致病机制,和脂质分子的变化可能导致视网膜组织损伤。然而,由该机制引起的视网膜脂质分布改变仍不清楚。因此，本研究采用视网膜缺血-再灌注模型分析假手术组和缺血-再灌注组之间的血脂变化.我们发现缺血再灌注损伤引起338个脂质分子的改变，这可能导致脂滴形成和线粒体损伤。值得注意的是,我们确定了各种脂质的特征性变化，包括胆固醇酯，心磷脂,还有神经酰胺,它可以作为评估视网膜损伤严重程度和治疗干预的潜在生物标志物。在这项研究中确定的缺血再灌注特异性特征提供了对这种病症的病理生理机制的更全面的理解。
