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Abstract：
BACKGROUND: The aim was to study aqueous humour inflammatory mediators\' levels in a cohort of Egyptian patients with diabetic macular oedema (DMO).
METHODS: This was a case-control prospective study conducted on 2 groups: 25 eyes of 22 (11 females) patients seeking treatment for DMO as patients group, and 10 eyes of 10 (4 females) cataract patients as a control group. Aqueous humour was aspirated before intravitreal injection (patients\' group) or cataract surgery (control group). Inflammatory mediators in aqueous humour were measured using a multiplex bead immunoassay kit of 27 pre-mixed cytokines.
RESULTS: Eotaxin, interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1/CCL2) and interleukin-8 (IL-8/CXCL8) were found significantly higher in patients\' group compared to control group (p = 0.043, 0.037, 0.001, 0.015 respectively). On the contrary, interferon-gamma (IFN-gamma) and granulocyte colony-stimulating factor (G-CSF) were found significantly higher in control group than patients\' group (p = 0.003, 0.019 respectively). Basic fibroblast growth factor (Basic-FGF/FGF-2) and interleukin-1 receptor antagonist (IL-1ra) were found higher (but not statistically significant) in controls (p = 0.100 and 0.070 respectively). Additionally, a negative and significant correlation was found between Eotaxin level in aqueous humour and central macular thickness.
CONCLUSIONS: Some mediators might be implicated in the pathogenesis of DMO either augmenting or suppressing role. Eotaxin, IP-10, MCP-1 and IL-8 might have a role in cases not responding to standard anti-vascular endothelial growth factor (VEGF) therapy. IL-1ra might have a protective role; therefore, the effectiveness of intravitreal injection of IL-1ra homologue needs to be studied in future clinical trials.
METHODS: This was a case-control prospective study conducted on 2 groups: 25 eyes of 22 (11 females) patients seeking treatment for DMO as patients group, and 10 eyes of 10 (4 females) cataract patients as a control group. Aqueous humour was aspirated before intravitreal injection (patients\' group) or cataract surgery (control group). Inflammatory mediators in aqueous humour were measured using a multiplex bead immunoassay kit of 27 pre-mixed cytokines.
RESULTS: Eotaxin, interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1/CCL2) and interleukin-8 (IL-8/CXCL8) were found significantly higher in patients\' group compared to control group (p = 0.043, 0.037, 0.001, 0.015 respectively). On the contrary, interferon-gamma (IFN-gamma) and granulocyte colony-stimulating factor (G-CSF) were found significantly higher in control group than patients\' group (p = 0.003, 0.019 respectively). Basic fibroblast growth factor (Basic-FGF/FGF-2) and interleukin-1 receptor antagonist (IL-1ra) were found higher (but not statistically significant) in controls (p = 0.100 and 0.070 respectively). Additionally, a negative and significant correlation was found between Eotaxin level in aqueous humour and central macular thickness.
CONCLUSIONS: Some mediators might be implicated in the pathogenesis of DMO either augmenting or suppressing role. Eotaxin, IP-10, MCP-1 and IL-8 might have a role in cases not responding to standard anti-vascular endothelial growth factor (VEGF) therapy. IL-1ra might have a protective role; therefore, the effectiveness of intravitreal injection of IL-1ra homologue needs to be studied in future clinical trials.
摘要：
背景：目的是研究一组埃及糖尿病性黄斑水肿（DMO）患者的房水炎症介质水平。
方法：这是一项病例对照前瞻性研究，对2组进行：22例（11例女性）寻求DMO治疗的患者的25只眼作为患者组，以10例(4例女性)白内障患者10只眼为对照组。在玻璃体内注射（患者组）或白内障手术（对照组）之前抽吸房水。使用27种预混合细胞因子的多重珠免疫测定试剂盒测量房水中的炎症介质。
结果：Eotaxin，干扰素γ诱导蛋白10（IP-10），与对照组相比，患者组的单核细胞趋化蛋白-1（MCP-1/CCL2）和白细胞介素-8（IL-8/CXCL8）显着升高（p=0.043，0.037，0.001，0.015）。相反,发现对照组的干扰素-γ（IFN-γ）和粒细胞集落刺激因子（G-CSF）明显高于患者组（p分别为0.003，0.019）。在对照组中发现碱性成纤维细胞生长因子（碱性FGF/FGF-2）和白介素-1受体拮抗剂（IL-1ra）较高（但无统计学意义）（分别为p=0.100和0.070）。此外,房水中的Eotaxin水平与中央黄斑厚度之间呈负相关。
结论：某些介质可能与DMO的发病机制有关，无论是增强还是抑制作用。eotaxin,IP-10，MCP-1和IL-8可能在对标准抗血管内皮生长因子（VEGF）疗法无反应的情况下发挥作用。IL-1ra可能具有保护作用；因此，玻璃体内注射IL-1ra同源物的有效性需要在未来的临床试验中进行研究.
方法：这是一项病例对照前瞻性研究，对2组进行：22例（11例女性）寻求DMO治疗的患者的25只眼作为患者组，以10例(4例女性)白内障患者10只眼为对照组。在玻璃体内注射（患者组）或白内障手术（对照组）之前抽吸房水。使用27种预混合细胞因子的多重珠免疫测定试剂盒测量房水中的炎症介质。
结果：Eotaxin，干扰素γ诱导蛋白10（IP-10），与对照组相比，患者组的单核细胞趋化蛋白-1（MCP-1/CCL2）和白细胞介素-8（IL-8/CXCL8）显着升高（p=0.043，0.037，0.001，0.015）。相反,发现对照组的干扰素-γ（IFN-γ）和粒细胞集落刺激因子（G-CSF）明显高于患者组（p分别为0.003，0.019）。在对照组中发现碱性成纤维细胞生长因子（碱性FGF/FGF-2）和白介素-1受体拮抗剂（IL-1ra）较高（但无统计学意义）（分别为p=0.100和0.070）。此外,房水中的Eotaxin水平与中央黄斑厚度之间呈负相关。
结论：某些介质可能与DMO的发病机制有关，无论是增强还是抑制作用。eotaxin,IP-10，MCP-1和IL-8可能在对标准抗血管内皮生长因子（VEGF）疗法无反应的情况下发挥作用。IL-1ra可能具有保护作用；因此，玻璃体内注射IL-1ra同源物的有效性需要在未来的临床试验中进行研究.
