Abstract：
Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.
To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.
Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.
Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.
NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation.
To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.
Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.
Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.
NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation.
摘要：
■胸腺细胞选择相关的高迁移率组框蛋白（TOX）和核受体4A（NR4A）的成员被称为参与T细胞衰竭的转录因子。
■评估急性白血病CD8+T细胞中TOX和NR4A1-3的mRNA表达。
从21名ALL患者和6名AML患者以及20名对照受试者获得血液样品。使用MACS分离CD8+T细胞。然后使用qRT-PCR评估TOX和NR4A1-3的相对基因表达。
■CD8+T细胞中TOXmRNA表达的比较在各研究组间无显著性差异(p>0.05),而NR4A1在AML患者中的表达显著低于对照组(p=0.0006)。此外，NR4A2和NR4A3的表达在ALL(分别为p=0.0049和p=0.0005)和AML(分别为p=0.0019和p=0.0055)患者中均显著降低.
■发现与对照组相比，AML和ALL患者的CD8+T细胞中NR4As表达较低，而TOX的mRNA表达无明显差异。尽管TOX和NR4As与实体瘤中的CD8+T细胞耗竭相关，它们可能在急性白血病中扮演不同的角色，这需要进一步调查。
■评估急性白血病CD8+T细胞中TOX和NR4A1-3的mRNA表达。
从21名ALL患者和6名AML患者以及20名对照受试者获得血液样品。使用MACS分离CD8+T细胞。然后使用qRT-PCR评估TOX和NR4A1-3的相对基因表达。
■CD8+T细胞中TOXmRNA表达的比较在各研究组间无显著性差异(p>0.05),而NR4A1在AML患者中的表达显著低于对照组(p=0.0006)。此外，NR4A2和NR4A3的表达在ALL(分别为p=0.0049和p=0.0005)和AML(分别为p=0.0019和p=0.0055)患者中均显著降低.
■发现与对照组相比，AML和ALL患者的CD8+T细胞中NR4As表达较低，而TOX的mRNA表达无明显差异。尽管TOX和NR4As与实体瘤中的CD8+T细胞耗竭相关，它们可能在急性白血病中扮演不同的角色，这需要进一步调查。
