{Reference Type}: Journal Article
{Title}: Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia.
{Author}: Mohammadi M;Asgarian-Omran H;Najafi B;Najafi A;Valadan R;Karami H;Naderisoraki M;Alizadeforoutan M;Shekarriz R;Tehrani M;
{Journal}: Iran J Immunol
{Volume}: 20
{Issue}: 4
{Year}: 2023 12 31
{Factor}: 1.206
{DOI}: 10.22034/iji.2023.97902.2537
{Abstract}: Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.<BR>To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.<BR>Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.<BR>Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.<BR>NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation.