PDF(Pubmed)
Abstract：
The immune functions of the body are intricately intertwined with the onset and advancement of tumors, and immunotherapy mediated by bioactive compounds has exhibited initial effectiveness in overcoming chemotherapy resistance and inhibiting tumor growth. However, the comprehensive interpretation of the roles played by immunologic components in the process of combating tumors remains to be elucidated. In this study, the Codonopsis pilosula glucofructan (CPG) prepared in our previous research was employed as an immunopotentiator, and the impacts of CPG on both the humoral and cellular immunity of S180 tumor-bearing mice were investigated. Results showed that CPG administration of 100 mg/kg could effectively inhibit tumor growth in mice with an inhibitory ratio of 45.37% and significantly improve the expression of Interleukin-2 (IL-2), Interferon-γ (IFN-γ), and Tumor Necrosis Factor-α (TNF-α). Additionally, CPG clearly enhanced B-cell-mediated humoral immunity and immune-cell-mediated cellular immunity, and, finally, induced S180 cell apoptosis by arresting cells in the G0/G1 phase, which might result from the IL-17 signaling pathway. These data may help to improve comprehension surrounding the roles of humoral and cellular immunity in anti-tumor immune responses.
摘要：
机体的免疫功能与肿瘤的发生和发展错综复杂地交织在一起,和由生物活性化合物介导的免疫疗法在克服化学疗法抗性和抑制肿瘤生长方面已显示出最初的有效性。然而,对免疫成分在抗肿瘤过程中的作用的全面解释仍有待阐明。在这项研究中,在我们以前的研究中制备的党参葡果聚糖（CPG）被用作免疫增强剂，研究了CPG对S180荷瘤小鼠体液免疫和细胞免疫的影响。结果表明，100mg/kg的CPG可有效抑制小鼠肿瘤生长，抑制率为45.37％，并显着提高白细胞介素-2（IL-2）的表达，干扰素-γ(IFN-γ),和肿瘤坏死因子-α（TNF-α）。此外,CPG明显增强B细胞介导的体液免疫和免疫细胞介导的细胞免疫，and,最后,通过将细胞阻滞在G0/G1期来诱导S180细胞凋亡，这可能是由IL-17信号通路引起的。这些数据可能有助于提高对体液和细胞免疫在抗肿瘤免疫应答中的作用的理解。
