PDF(Pubmed)
Abstract：
The copper transporter (COPT/Ctr) gene family plays a critical part in maintaining the balance of the metal, and many diverse species depend on COPT to move copper (Cu) across the cell membrane. In Arabidopsis thaliana, Oryza sativa, Medicago sativa, Zea mays, Populus trichocarpa, Vitis vinifera, and Solanum lycopersicum, a genome-wide study of the COPT protein family was performed. To understand the major roles of the COPT gene family in Kandelia obovata (Ko), a genome-wide study identified four COPT genes in the Kandelia obovata genome for the first time. The domain and 3D structural variation, phylogenetic tree, chromosomal distributions, gene structure, motif analysis, subcellular localization, cis-regulatory elements, synteny and duplication analysis, and expression profiles in leaves and Cu were all investigated in this research. Structural and sequence investigations show that most KoCOPTs have three transmembrane domains (TMDs). According to phylogenetic research, these KoCOPTs might be divided into two subgroups, just like Populus trichocarpa. KoCOPT gene segmental duplications and positive selection pressure were discovered by universal analysis. According to gene structure and motif analysis, most KoCOPT genes showed consistent exon-intron and motif organization within the same group. In addition, we found five hormones and four stress- and seven light-responsive cis-elements in the KoCOPTs promoters. The expression studies revealed that all four genes changed their expression levels in response to copper (CuCl2) treatments. In summary, our study offers a thorough overview of the Kandelia obovata COPT gene family\'s expression pattern and functional diversity, making it easier to characterize each KoCOPT gene\'s function in the future.
摘要：
铜转运体(COPT/Ctr)基因家族在维持金属平衡中起着至关重要的作用,许多不同的物种依赖于COPT来移动铜（Cu）穿过细胞膜。在拟南芥中，水稻,紫花苜蓿,ZeaMays,毛果杨，葡萄，和番茄红素,我们对COPT蛋白家族进行了全基因组研究.为了了解COPT基因家族在Kandeliaobovata（Ko）中的主要作用，一项全基因组研究首次确定了Kandeliaobovata基因组中的四个COPT基因。域和3D结构变化，系统发育树，染色体分布,基因结构，主题分析，亚细胞定位，顺式调控要素，同义和重复分析，在这项研究中，研究了叶片和Cu的表达谱。结构和序列研究表明，大多数KoCOPT具有三个跨膜结构域（TMD）。根据系统发育研究,这些KoCOPT可能分为两个子组，就像毛果杨树一样.通过通用分析发现了KoCOPT基因片段重复和正选择压力。根据基因结构和基序分析,大多数KoCOPT基因在同一组内显示一致的外显子-内含子和基序组织。此外,我们在KoCOPTs启动子中发现了五种激素和四种应激和七种光响应顺式元件。表达研究显示,所有四个基因响应于铜(CuCl2)处理而改变其表达水平。总之,我们的研究提供了一个全面的概述kandeliaobovataCOPT基因家族的表达模式和功能多样性，使得将来更容易表征每个KoCOPT基因的功能。
