Abstract：
Phytophthora sojae, one of the most devastating Oomycete pathogens, causes severe diseases that lead to economic loss in the soybean industry. The production of zoospores play a crucial role during the development of Phytophthora disease. In this work, CRISPR/Cas9 genome editing technology were used to obtain protein kinase A regulatory subunit (PsPkaR) knockout mutants. The role of PsPkaR in the production of zoospores and pathogenicity of P. sojae was analyzed. The overall findings indicate that PsPkaR is involved in regulating the growth process of P. sojae, primarily affecting the hyphal morphology and growth rate. Additionally, PsPkaR participates in the regulation of the release process of zoospores. Specifically, knocking-out PsPkaR resulted in incomplete cytoplasmic differentiation and uneven protoplast division, leading to abnormal release of zoospores. Furthermore, when the PsPkaR knockout mutants were inoculated on soybean leaves, the pathogenicity was significantly reduced compared to that of the wild-type and control strains. These findings of this study provide important clues and evidence regarding the role of the cAMP-PKA signaling pathway in the interaction between P. sojae and its host. This work contributes to a better understanding of the pathogenic mechanism of P. sojae and the development of corresponding prevention and control strategies.
摘要：
大豆疫霉,最具破坏性的卵菌病原体之一，造成严重的疾病，导致大豆产业的经济损失。游动孢子的产生在疫霉病的发展中起着至关重要的作用。在这项工作中,利用CRISPR/Cas9基因组编辑技术获得蛋白激酶A调节亚基(PsPkaR)敲除突变体。分析了PsPkaR在游动孢子产生中的作用和大豆芽孢杆菌的致病性。总体结果表明，PsPkaR参与调节大豆的生长过程，主要影响菌丝形态和生长速率。此外,PsPkaR参与动物孢子释放过程的调节。具体来说,敲除PsPkaR导致细胞质分化不完全和原生质体分裂不均，导致游动孢子的异常释放。此外，当PsPkaR敲除突变体接种在大豆叶片上时，与野生型和对照菌株相比，致病性显着降低。本研究的这些发现为cAMP-PKA信号通路在大豆及其宿主相互作用中的作用提供了重要的线索和证据。这项工作有助于更好地了解大豆疫霉的致病机制并制定相应的预防和控制策略。
