Abstract：
In this study, in order to reveal the immune response against the disease in naturally infected sheep with SPPV, the expressions of various pro- or anti-inflammatory cytokines such as tumour necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), interleukin-1beta (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) and interleukin-12 (IL-12) were evaluated immunohistochemically. The material of this study consisted of tissue samples taken from 24 sheep, which were brought as dead for routine histopathological examination to the Department of Pathology. Avidin-biotin-peroxidase method was used for immunohistochemistry. Characteristic pox lesions were observed in the skin, lungs and kidneys. In histopathological examinations, pox cells, which are very characteristic for the diagnosis of the disease, were observed in all three tissues. Capripoxvirus nucleic acid was detected in 8 of the 24 tissues. Samples were sequenced, and a phylogenetic tree was constructed with reference strains from GenBank. Strains from the study clustered with sheeppox virus references. In conclusion, the levels of pro-inflammatory cytokines such as TNF-α, IFN-γ, IL-1β, IL-2, IL-8 and IL12 (Th1) were much more dominant compared to the levels of anti-inflammatory cytokines: IL-10 and IL-6 (Th2). This supported the fact that the cellular immune response is much more effective than the humoral immune response in sheeppox.
摘要：
在这项研究中,为了揭示SPPV自然感染绵羊对该病的免疫反应，各种促炎或抗炎细胞因子如肿瘤坏死因子α(TNF-α)的表达,干扰素γ（IFN-γ），白细胞介素-1β（IL-1β），白细胞介素-2（IL-2），白细胞介素-6（IL-6），白细胞介素-8（IL-8），对白细胞介素-10（IL-10）和白细胞介素-12（IL-12）进行免疫组织化学评估。这项研究的材料包括取自24只绵羊的组织样本，将其作为常规组织病理学检查的死亡带到病理学系。抗生物素蛋白-生物素-过氧化物酶方法用于免疫组织化学。在皮肤中观察到特征性的痘痘病变，肺和肾.在组织病理学检查中，痘痘细胞,这是疾病诊断的典型特征，在所有三个组织中都观察到。在24个组织中的8个组织中检测到Capropoxvirus核酸。对样品进行测序，并用GenBank的参考菌株构建了系统发育树。来自该研究的菌株与羊痘病毒参考文献聚集在一起。总之,促炎细胞因子的水平，如TNF-α，IFN-γ，IL-1β,与抗炎细胞因子IL-10和IL-6（Th2）的水平相比，IL-2，IL-8和IL12（Th1）的优势更大。这支持了羊痘中的细胞免疫应答比体液免疫应答有效得多的事实。
