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Abstract：
Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis. This protocol presents time-lapse imaging of morphological changes during pyroptosis using a confocal microscope. We describe the step-by-step ectopic expression of gasdermin, cell staining with nuclear and membrane probes, and visualization of pyroptosis by time-lapse imaging. This protocol is applicable to monitoring pyroptosis in various situations. For complete details on the use and execution of this protocol, please refer to Qin et al. (2023).1.
摘要：
发热是一种由gasdermin引起的调节坏死。渗透细胞肿胀和膜穿孔是焦亡的关键特征。该协议提出了使用共聚焦显微镜在焦度过程中形态变化的延时成像。我们描述了gasdermin的逐步异位表达，用核和膜探针进行细胞染色，和通过延时成像可视化焦度。该协议适用于监测各种情况下的焦亡。有关此协议的使用和执行的完整详细信息，请参考秦等人。（2023）.1。
