%0 Journal Article
%T Time-lapse live-cell imaging of pyroptosis by confocal microscopy.
%A Jiang S
%A Qin K
%A Sun L
%J STAR Protoc
%V 4
%N 4
%D 2023 Dec 15
%M 37948183
暂无%R 10.1016/j.xpro.2023.102708
%X Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis. This protocol presents time-lapse imaging of morphological changes during pyroptosis using a confocal microscope. We describe the step-by-step ectopic expression of gasdermin, cell staining with nuclear and membrane probes, and visualization of pyroptosis by time-lapse imaging. This protocol is applicable to monitoring pyroptosis in various situations. For complete details on the use and execution of this protocol, please refer to Qin et al. (2023).1.