PDF(Pubmed)
Abstract：
Irritable bowel syndrome (IBS) is a prevalent gastrointestinal (GI) tract disorder. Although the main reason for IBS is not clear, the interaction between intestinal microorganisms and the gut barrier seems to play an important role in pathogenesis of IBS. The current study aimed to investigate the effect of Blastocystis on the gut microbiota profile and the circulation levels of microRNA (mir)-16 of IBS patients compared to healthy subjects. Stool and blood samples were collected from 80 participants including 40 samples from each IBS and healthy group. Upon DNA extraction from stool samples, barcoding region and quantitative real-time PCR were analyzed to investigate Blastocystis and the microbiota profile, respectively. RNA was extracted from serum samples of included subjects and the expression of mir-16 was evaluated using stem-loop protocol and qreal-time PCR. Significant changes between IBS patients and healthy controls was observed in Firmicutes, Actinobacteria, Faecalibacterium, and Alistipes. In IBS patients, the relative abundance of Bifidobacteria was directly correlated with the presence of Blastocystis, while Alistipes was decreased with Blastocystis. Lactobacillus was significantly increased in Blastocystis carriers. In healthy subjects, the relative abundance of Bifidobacteria was decreased, but Alistipes was increased in Blastocystis carriers. The changes in the Firmicutes/Bacteroidetes ratio was not significant in different groups. The relative expression of mir-16 in Blastocystis-negative IBS patients and healthy carriers was significantly overexpressed compared to control group. The presence of Blastocystis, decreased the relative expression of mir-16 in IBS patients compared to Blastocystis-negative IBS patients. The present study revealed that Blastocystis has the ability to change the abundance of some phyla/genera of bacteria in IBS and healthy subjects. Moreover, Blastocystis seems to modulate the relative expression of microRNAs to control the gut atmosphere, apply its pathogenicity, and provide a favor niche for its colonization.
摘要：
肠易激综合征（IBS）是一种常见的胃肠道（GI）疾病。虽然IBS的主要原因尚不清楚，肠道微生物与肠道屏障的相互作用在IBS的发病机制中起着重要作用。目前的研究旨在研究囊胚对IBS患者的肠道微生物群分布和microRNA（mir）-16循环水平的影响。从80名参与者中收集粪便和血液样本，包括来自每个IBS和健康组的40个样本。从粪便样本中提取DNA后，条形码区和定量实时PCR分析，以研究囊胚和微生物区系，分别。从包括的受试者的血清样品中提取RNA,并使用茎-环方案和qreal-timePCR评估mir-16的表达。在Firmicutes中观察到IBS患者和健康对照之间的显着变化，放线菌,粪杆菌,和Alistipes。在IBS患者中，双歧杆菌的相对丰度与胚泡的存在直接相关，而囊性囊虫则减少。乳杆菌在囊胚携带者中显著增加。在健康的受试者中，双歧杆菌的相对丰度降低，但芽囊原虫携带者的Alistipes增加。Firmicutes/拟杆菌比率在不同组中的变化不显著。与对照组相比，mir-16在胚泡阴性IBS患者和健康携带者中的相对表达明显过表达。囊胚的存在，与胚泡阴性IBS患者相比，IBS患者中mir-16的相对表达降低。本研究表明，囊胚具有改变IBS和健康受试者中某些门/属细菌丰度的能力。此外，囊胚似乎调节microRNAs的相对表达以控制肠道环境，应用其致病性，并为其殖民提供有利的利基。
