PDF(Pubmed)
Abstract：
Kaposi sarcoma (KS), a multifocal vascular neoplasm frequently observed in HIV-positive individuals, primarily affects the skin, mucous membranes, visceral organs, and lymph nodes. KS is associated primarily with Kaposi sarcoma-associated herpesvirus (KSHV) infection. In this case report, we present a rare occurrence of co-infection and co-localization of KSHV and Epstein-Barr virus (EBV) in KS arising from the conjunctiva, which, to our knowledge, has not been reported previously. Immunohistochemistry (IHC), DNA polymerase chain reaction (PCR), and EBV-encoded RNA in situ hybridization (EBER-ISH) were utilized to demonstrate the presence of KSHV and EBV infection in the ocular KS lesion. Nearly all KSHV-positive cells displayed co-infection with EBV. In addition, the KS lesion revealed co-localization of KSHV Latency-Associated Nuclear Antigen (LANA) and EBV Epstein Barr virus Nuclear Antigen-1 (EBNA1) by multi-colored immunofluorescence staining with different anti-EBNA1 antibodies, indicating the possibility of interactions between these two gamma herpesviruses within the same lesion. Additional study is needed to determine whether EBV co-infection in KS is a common or an opportunistic event that might contribute to KS development and progression.
摘要：
卡波西肉瘤(KS),在HIV阳性个体中经常观察到的多灶性血管肿瘤，主要影响皮肤，粘膜,内脏器官,和淋巴结。KS主要与卡波西肉瘤相关疱疹病毒(KSHV)感染有关。在这个案例报告中,我们提出了一种罕见的共同感染和共同定位的KSHV和EB病毒（EBV）在KS中引起的结膜，which,根据我们的知识，以前没有报道过。免疫组织化学(IHC),DNA聚合酶链反应（PCR），和EBV编码的RNA原位杂交（EBER-ISH）用于证明眼部KS病变中KSHV和EBV感染的存在。几乎所有KSHV阳性细胞都显示与EBV共感染。此外,KS病变通过不同抗EBNA1抗体的多色免疫荧光染色显示KSHV潜伏相关核抗原（LANA）和EBV爱泼斯坦巴尔病毒核抗原-1（EBNA1）的共定位，表明这两种γ疱疹病毒在同一病变内相互作用的可能性。需要进一步的研究来确定KS中EBV共感染是常见的还是可能导致KS发生和进展的机会性事件。
