Abstract：
Dysregulation of gene expression is critical for the progression of cancer. The augmented expression of hnRNP A1 in patients with hepatocellular carcinoma (HCC) has been related to its oncogenic functions. However, the underlying mechanisms responsible for upregulation of hnRNP A1 have not been fully elucidated. In the present study, we identified microRNA-195-5p (miR-195-5p), a miRNA downregulated in HCC, as a novel regulator governing hnRNP A1 expression. Notably, our investigations showed an inverse correlation between hnRNP A1 level, which was increased in HCC, and miR-195-5p level, which was decreased. Our findings demonstrated that hnRNP A1 significantly enhanced the migration and invasion of PLC/PRF/5 cells through its association with mRNAs regulating metastasis. MiR-195-5p also interfered with the hnRNP A1-mediated cell migration by targeting hnRNP A1. Our results underscore the significance of the miR-195-5p/hnRNP A1 axis in regulating the migratory potential of cancer cells and its role in promoting HCC by orchestrating cell migration processes.
摘要：
基因表达失调对癌症的进展至关重要。hnRNPA1在肝细胞癌（HCC）患者中的表达增强与其致癌功能有关。然而,hnRNPA1上调的潜在机制尚未完全阐明.在本研究中,我们鉴定了microRNA-195-5p（miR-195-5p），在HCC中下调的miRNA，作为控制hnRNPA1表达的新型调节剂。值得注意的是,我们的调查显示，hnRNPA1水平之间呈负相关，在HCC中增加了，和miR-195-5p水平，减少了。我们的发现表明，hnRNPA1通过与调节转移的mRNA相关联，显着增强了PLC/PRF/5细胞的迁移和侵袭。MiR-195-5p还通过靶向hnRNPA1干扰hnRNPA1介导的细胞迁移。我们的结果强调了miR-195-5p/hnRNPA1轴在调节癌细胞的迁移潜力及其通过协调细胞迁移过程促进HCC中的作用中的意义。
