Abstract：
Vitamin D has received increasing attention because of its association with atopic disease development. Limited studies that have been done on the impact of maternal vitamin D levels during pregnancy on infantile eczema are still debatable. We wanted to discover the effect of maternal vitamin D on infantile eczema and explore whether regulatory T cells (Treg) play a role in this process. 219 pairs of mothers and children were enrolled. Maternal fasting venous blood was collected in pregnancy\'s second and third trimesters to determine vitamin D levels. Cord blood and placenta samples were collected during childbirth for detecting levels of genes, proteins and cytokines. Pediatricians followed up the prevalence of eczema in infants within 1 year. The reported rate of vitamin D deficiency and insufficiency was 35.6% and 28.3%. Lower maternal 25(OH)D3 levels were related to a higher risk of infantile eczema. Foxp3 gene expression is lower in cord blood of infants with eczema compared to infants without eczema. There was a positive correlation between maternal 25(OH)D3 levels and the expression of FOXP3 gene in cord blood. Compared to vitamin D sufficiency women, vitamin D deficiency women\'s placental FOXP3 protein expression was decreased and PI3K/AKT/mTOR protein was up-regulated. Our study demonstrates that low prenatal maternal vitamin D levels increased the risk of infantile eczema aged 0-1 year, which might be related to the downregulating of the FOXP3 gene expression in cord blood and decreased placental FOXP3 protein expression. Low placental FOXP3 protein was related with activating PI3K/AKT/mTOR signaling pathway.
摘要：
维生素D由于其与特应性疾病的发展有关而受到越来越多的关注。关于怀孕期间母体维生素D水平对婴儿湿疹的影响的有限研究仍然存在争议。我们想发现母体维生素D对婴儿湿疹的影响，并探索调节性T细胞（Treg）是否在此过程中发挥作用。219对母亲和儿童被录取。在妊娠中期和中期收集孕妇空腹静脉血以确定维生素D水平。在分娩期间收集脐带血和胎盘样本以检测基因水平，蛋白质和细胞因子。儿科医生随访1年内婴儿湿疹的患病率。维生素D缺乏和不足的报告率为35.6％和28.3％。母亲25（OH）D3水平较低与婴儿湿疹的风险较高有关。与没有湿疹的婴儿相比，湿疹婴儿的脐带血中Foxp3基因表达较低。母体25(OH)D3水平与脐血FOXP3基因表达呈正相关。与维生素D充足的女性相比，维生素D缺乏妇女胎盘FOXP3蛋白表达降低，PI3K/AKT/mTOR蛋白上调。我们的研究表明，低孕妇产前维生素D水平会增加0-1岁婴儿湿疹的风险，这可能与下调脐血FOXP3基因表达和降低胎盘FOXP3蛋白表达有关。胎盘FOXP3蛋白水平降低与PI3K/AKT/mTOR信号通路激活有关。
