PDF(Pubmed)
Abstract：
UNASSIGNED: Intelligent hydrogels continue to encounter formidable obstacles in the field of cancer treatment. A wide variety of hydrogel materials have been designed for diverse purposes, but materials with satisfactory therapeutic effects are still urgently needed.
UNASSIGNED: Here, we prepared an injectable hydrogel by means of physical crosslinking. Carbon nanoparticle suspension injection (CNSI), a sentinel lymph node imaging agent that has been widely used in the clinic, with sodium β-glycerophosphate (β-GP) were added to a temperature-sensitive chitosan (CS) hydrogel (CS/GP@CN) as an agent for photothermal therapy (PTT). After evaluating the rheological, morphological, and structural properties of the hydrogel, we used 4T1 mouse breast cancer cells and B16 melanoma cells to assess its in vitro properties. Then, we intratumorally injected the hydrogel into BALB/c tumor-bearing mice to assess the in vivo PTT effect, antitumor immune response and the number of lung metastases.
UNASSIGNED: Surprisingly, this nanocarbon hydrogel called CS/GP@CN hydrogel not only had good biocompatibility and a great PTT effect under 808nm laser irradiation but also facilitated the maturation of dendritic cells to stimulate the antitumor immune response and had an extraordinary antimetastatic effect in the lungs.
UNASSIGNED: Overall, this innovative temperature-sensitive nanocarbon hydrogel, which exists in a liquid state at room temperature and transforms to a gel at 37 °C, is an outstanding local delivery platform with tremendous PTT potential and broad clinical application prospects.
UNASSIGNED: Here, we prepared an injectable hydrogel by means of physical crosslinking. Carbon nanoparticle suspension injection (CNSI), a sentinel lymph node imaging agent that has been widely used in the clinic, with sodium β-glycerophosphate (β-GP) were added to a temperature-sensitive chitosan (CS) hydrogel (CS/GP@CN) as an agent for photothermal therapy (PTT). After evaluating the rheological, morphological, and structural properties of the hydrogel, we used 4T1 mouse breast cancer cells and B16 melanoma cells to assess its in vitro properties. Then, we intratumorally injected the hydrogel into BALB/c tumor-bearing mice to assess the in vivo PTT effect, antitumor immune response and the number of lung metastases.
UNASSIGNED: Surprisingly, this nanocarbon hydrogel called CS/GP@CN hydrogel not only had good biocompatibility and a great PTT effect under 808nm laser irradiation but also facilitated the maturation of dendritic cells to stimulate the antitumor immune response and had an extraordinary antimetastatic effect in the lungs.
UNASSIGNED: Overall, this innovative temperature-sensitive nanocarbon hydrogel, which exists in a liquid state at room temperature and transforms to a gel at 37 °C, is an outstanding local delivery platform with tremendous PTT potential and broad clinical application prospects.
摘要：
■智能水凝胶在癌症治疗领域继续遇到巨大的障碍。各种各样的水凝胶材料已经被设计用于不同的目的，但是仍然迫切需要具有令人满意的治疗效果的材料。
■这里，我们通过物理交联制备了可注射的水凝胶。碳纳米颗粒悬浮注射（CNSI），一种广泛用于临床的前哨淋巴结显像剂，将β-甘油磷酸钠（β-GP）添加到温度敏感的壳聚糖（CS）水凝胶（CS/GP@CN）中作为光热疗法（PTT）的试剂。在评估流变学后，形态学,和水凝胶的结构特性，我们使用4T1小鼠乳腺癌细胞和B16黑色素瘤细胞来评估其体外特性。然后，我们将水凝胶瘤内注射到BALB/c荷瘤小鼠体内以评估体内PTT效应，抗肿瘤免疫反应和肺转移瘤的数量。
■令人惊讶的是，这种称为CS/GP@CN水凝胶的纳米碳水凝胶不仅在808nm激光照射下具有良好的生物相容性和良好的PTT效应，而且还促进了树突状细胞的成熟以刺激抗肿瘤免疫反应，并在肺部具有非凡的抗转移作用。
■总的来说，这种创新的温度敏感纳米碳水凝胶，它在室温下以液态存在，并在37°C时转化为凝胶，是一种优秀的局部给药平台，具有巨大的PTT潜力和广阔的临床应用前景。
■这里，我们通过物理交联制备了可注射的水凝胶。碳纳米颗粒悬浮注射（CNSI），一种广泛用于临床的前哨淋巴结显像剂，将β-甘油磷酸钠（β-GP）添加到温度敏感的壳聚糖（CS）水凝胶（CS/GP@CN）中作为光热疗法（PTT）的试剂。在评估流变学后，形态学,和水凝胶的结构特性，我们使用4T1小鼠乳腺癌细胞和B16黑色素瘤细胞来评估其体外特性。然后，我们将水凝胶瘤内注射到BALB/c荷瘤小鼠体内以评估体内PTT效应，抗肿瘤免疫反应和肺转移瘤的数量。
■令人惊讶的是，这种称为CS/GP@CN水凝胶的纳米碳水凝胶不仅在808nm激光照射下具有良好的生物相容性和良好的PTT效应，而且还促进了树突状细胞的成熟以刺激抗肿瘤免疫反应，并在肺部具有非凡的抗转移作用。
■总的来说，这种创新的温度敏感纳米碳水凝胶，它在室温下以液态存在，并在37°C时转化为凝胶，是一种优秀的局部给药平台，具有巨大的PTT潜力和广阔的临床应用前景。
