Abstract：
UNASSIGNED: To evaluate the effects of combined treatment with tannic acid and ferric ions on the biomechanical and anti-calcification properties of glutaraldehyde-fixed bovine jugular veins after xenografting.
UNASSIGNED: Two-point bending test and uniaxial tensile test were used to evaluate the flexural and biomechanical properties; Subcutaneous implantation in rat and right ventricular outflow tract reconstruction of sheep were used to evaluate the anti-calcification effects; The performance of the graft in sheep models was evaluated every month after the surgery with echocardiography examination. Markers of macrophages, T lymphocytes, smooth muscle cell osteogenic differentiation and matrix metalloproteinases in sheep explants were detected by immunohistochemistry.
UNASSIGNED: The flexibility of the bovine jugular veins cotreated with ferric ions-tannic acid was improved while maintaining biomechanical properties and excellent anti-calcification effects. Echocardiography results showed that the grafts functioned well in the animals without stenosis or reflux of the valve. Immunohistochemical studies showed that the osteogenic differentiation marker (Runx2) was detected in calcified regions and colocalised with the SMC marker (α-SMA). Compared to the glutaraldehyde-treated samples, T-cell marker (CD3), matrix metalloproteinase-2 and 9 expressions were reduced in the ferric ions-tannic acid treated group.
UNASSIGNED: Ferric ions-tannic acid treatment can give the conduits better flexibility with excellent biomechanical properties and anti-calcification effects, making it a promising bovine jugular veins processing method.
UNASSIGNED: Two-point bending test and uniaxial tensile test were used to evaluate the flexural and biomechanical properties; Subcutaneous implantation in rat and right ventricular outflow tract reconstruction of sheep were used to evaluate the anti-calcification effects; The performance of the graft in sheep models was evaluated every month after the surgery with echocardiography examination. Markers of macrophages, T lymphocytes, smooth muscle cell osteogenic differentiation and matrix metalloproteinases in sheep explants were detected by immunohistochemistry.
UNASSIGNED: The flexibility of the bovine jugular veins cotreated with ferric ions-tannic acid was improved while maintaining biomechanical properties and excellent anti-calcification effects. Echocardiography results showed that the grafts functioned well in the animals without stenosis or reflux of the valve. Immunohistochemical studies showed that the osteogenic differentiation marker (Runx2) was detected in calcified regions and colocalised with the SMC marker (α-SMA). Compared to the glutaraldehyde-treated samples, T-cell marker (CD3), matrix metalloproteinase-2 and 9 expressions were reduced in the ferric ions-tannic acid treated group.
UNASSIGNED: Ferric ions-tannic acid treatment can give the conduits better flexibility with excellent biomechanical properties and anti-calcification effects, making it a promising bovine jugular veins processing method.
摘要：
■评估单宁酸和三价铁离子联合治疗对异种移植后戊二醛固定的牛颈静脉的生物力学和抗钙化特性的影响。
■采用两点弯曲试验和单轴拉伸试验评价其弯曲和生物力学性能；采用大鼠皮下植入和羊右室流出道重建术评价其抗钙化作用；术后每个月用超声心动图评价移植体在羊模型中的表现。巨噬细胞的标记，T淋巴细胞,免疫组织化学法检测绵羊外植体平滑肌细胞成骨分化和基质金属蛋白酶。
■铁离子-单宁酸共混的牛颈静脉的柔韧性得到改善，同时保持了生物力学特性和出色的抗钙化作用。超声心动图结果表明，移植物在动物中功能良好，无狭窄或瓣膜回流。免疫组织化学研究表明，在钙化区域检测到成骨分化标记（Runx2），并与SMC标记（α-SMA）共定位。与戊二醛处理的样品相比，T细胞标记（CD3），三价铁离子-单宁酸处理组基质金属蛋白酶-2和9的表达降低。
■铁离子-单宁酸处理可使导管具有更好的柔韧性，具有出色的生物力学特性和抗钙化作用，使其成为一种有前途的牛颈静脉处理方法。
■采用两点弯曲试验和单轴拉伸试验评价其弯曲和生物力学性能；采用大鼠皮下植入和羊右室流出道重建术评价其抗钙化作用；术后每个月用超声心动图评价移植体在羊模型中的表现。巨噬细胞的标记，T淋巴细胞,免疫组织化学法检测绵羊外植体平滑肌细胞成骨分化和基质金属蛋白酶。
■铁离子-单宁酸共混的牛颈静脉的柔韧性得到改善，同时保持了生物力学特性和出色的抗钙化作用。超声心动图结果表明，移植物在动物中功能良好，无狭窄或瓣膜回流。免疫组织化学研究表明，在钙化区域检测到成骨分化标记（Runx2），并与SMC标记（α-SMA）共定位。与戊二醛处理的样品相比，T细胞标记（CD3），三价铁离子-单宁酸处理组基质金属蛋白酶-2和9的表达降低。
■铁离子-单宁酸处理可使导管具有更好的柔韧性，具有出色的生物力学特性和抗钙化作用，使其成为一种有前途的牛颈静脉处理方法。
