PDF(Pubmed)
Abstract：
UNASSIGNED: Supplemented Erzhi Wan (SEZW) is a Traditional Chinese Medicine commonly used in the treatment of androgenetic alopecia (AGA). This study aims to verify the effectiveness of SEZW for the treatment of AGA in mice and explore the potential molecular mechanisms underlying its function using network pharmacology and molecular docking.
UNASSIGNED: Forty mice were divided into five groups: Control, AGA-model, AGA-Positive, SEZW Low Dose, and SEZW High Dose. Hair regrowth in mice was evaluated by scoring hair on days 0, 14, and 28 post-treatment and weighing mouse hair on day 28 post-treatment. The targets of the active compounds of SEZW were obtained using the Traditional Chinese Medicine Database. AGA-related targets were downloaded from five databases. Then, the overlapping genes were identified. A protein-protein interaction network was constructed using the STRING database. Hub targets were determined through analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Finally, molecular docking of active compounds and hub targets was performed.
UNASSIGNED: Hair regrowth in mice in the SEZW treatment groups was significantly enhanced relative to that in the AGA-model mice. A total of 59 potential drug-disease targets were identified. Based on the GO/KEGG analysis results, oxidative stress and gland development were identified as potential mechanisms of action of SEZW in AGA treatment. The PI3K-Akt and AGE-RAGE signaling pathways and seven hub targets were identified as the potential underlying mechanism of SEZW function. Molecular docking results showed that the most active SEZW compounds bind stably to several of the candidate disease targets.
UNASSIGNED: SEZW is effective in the treatment of AGA in a mouse model. Combined with network pharmacological analysis, the potential mechanisms, signaling pathways, and hub targets of SEZW in the treatment of AGA were identified, providing new ideas for further studies.
UNASSIGNED: Forty mice were divided into five groups: Control, AGA-model, AGA-Positive, SEZW Low Dose, and SEZW High Dose. Hair regrowth in mice was evaluated by scoring hair on days 0, 14, and 28 post-treatment and weighing mouse hair on day 28 post-treatment. The targets of the active compounds of SEZW were obtained using the Traditional Chinese Medicine Database. AGA-related targets were downloaded from five databases. Then, the overlapping genes were identified. A protein-protein interaction network was constructed using the STRING database. Hub targets were determined through analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Finally, molecular docking of active compounds and hub targets was performed.
UNASSIGNED: Hair regrowth in mice in the SEZW treatment groups was significantly enhanced relative to that in the AGA-model mice. A total of 59 potential drug-disease targets were identified. Based on the GO/KEGG analysis results, oxidative stress and gland development were identified as potential mechanisms of action of SEZW in AGA treatment. The PI3K-Akt and AGE-RAGE signaling pathways and seven hub targets were identified as the potential underlying mechanism of SEZW function. Molecular docking results showed that the most active SEZW compounds bind stably to several of the candidate disease targets.
UNASSIGNED: SEZW is effective in the treatment of AGA in a mouse model. Combined with network pharmacological analysis, the potential mechanisms, signaling pathways, and hub targets of SEZW in the treatment of AGA were identified, providing new ideas for further studies.
摘要：
■补充二治丸（SEZW）是一种常用于治疗雄激素性脱发（AGA）的传统中药。本研究旨在验证SEZW治疗小鼠AGA的有效性，并利用网络药理学和分子对接技术探索其潜在的分子机制。
■将40只小鼠分为五组：对照组，AGA模型，AGA-阳性,SEZW低剂量,和SEZW高剂量。通过在治疗后第0、14和28天对毛发进行评分并在治疗后第28天对小鼠毛发进行称重来评估小鼠的毛发再生。使用中药数据库获得了SEZW的活性化合物的目标。从五个数据库下载与AGA相关的目标。然后，鉴定了重叠的基因。使用STRING数据库构建蛋白质-蛋白质相互作用网络。通过分析确定了枢纽目标。进行基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径富集分析。最后,进行活性化合物和hub靶标的分子对接。
■SEZW治疗组小鼠的毛发再生相对于AGA模型小鼠显著增强。总共确定了59个潜在的药物-疾病靶标。根据GO/KEGG分析结果，氧化应激和腺体发育被确定为SEZW在AGA治疗中的潜在作用机制。PI3K-Akt和AGE-RAGE信号通路和7个枢纽靶标被鉴定为SEZW功能的潜在潜在潜在机制。分子对接结果显示,最具活性的SEZW化合物稳定地结合几个候选疾病靶标。
■SEZW在小鼠模型中有效治疗AGA。结合网络药理分析,潜在的机制，信号通路，并确定了SEZW在AGA治疗中的枢纽靶标，为进一步研究提供新的思路。
■将40只小鼠分为五组：对照组，AGA模型，AGA-阳性,SEZW低剂量,和SEZW高剂量。通过在治疗后第0、14和28天对毛发进行评分并在治疗后第28天对小鼠毛发进行称重来评估小鼠的毛发再生。使用中药数据库获得了SEZW的活性化合物的目标。从五个数据库下载与AGA相关的目标。然后，鉴定了重叠的基因。使用STRING数据库构建蛋白质-蛋白质相互作用网络。通过分析确定了枢纽目标。进行基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径富集分析。最后,进行活性化合物和hub靶标的分子对接。
■SEZW治疗组小鼠的毛发再生相对于AGA模型小鼠显著增强。总共确定了59个潜在的药物-疾病靶标。根据GO/KEGG分析结果，氧化应激和腺体发育被确定为SEZW在AGA治疗中的潜在作用机制。PI3K-Akt和AGE-RAGE信号通路和7个枢纽靶标被鉴定为SEZW功能的潜在潜在潜在机制。分子对接结果显示,最具活性的SEZW化合物稳定地结合几个候选疾病靶标。
■SEZW在小鼠模型中有效治疗AGA。结合网络药理分析,潜在的机制，信号通路，并确定了SEZW在AGA治疗中的枢纽靶标，为进一步研究提供新的思路。
