PDF(Pubmed)
Abstract：
Adult Amblyomma sculptum and Amblyomma aureolatum ticks are partially refractory to Rickettsia rickettsii when fed on infected hosts, hindering the functional characterization of potentially protective targets in the bacterial acquisition. In the current study, we used the anal pore route to infect adult A. sculptum and A. aureolatum ticks with R. rickettsii and to assess the effects of the knockdown of microplusin in infection control. The anal pore route was efficient to infect both species, resulting in a prevalence of around 100% of infected ticks. Higher loads of R. rickettsii were detected in microplusin-silenced A. aureolatum in relation to the control, as previously obtained when microplusin-silenced ticks were fed on R. rickettsii-infected rabbits. This is the first report showing R. rickettsii infection through the anal pore in Amblyomma ticks, highlighting this route as a powerful tool to assess the role played by additional targets in the control of pathogens.
摘要：
当在受感染的宿主上喂食时，成年的弱球雕塑和黄球蜱对立克次体是部分难治的，阻碍细菌采集中潜在保护性靶标的功能表征。在目前的研究中,我们使用肛门毛孔途径用R.rickettsii感染成年A.sculptum和A.aureolatumtick，并评估了抑制microplusin在感染控制中的作用。肛门孔途径能有效感染这两个物种，导致约100％的感染蜱的患病率。与对照组相比，在沉默的微plusinA.aureolatum中检测到较高的立克次体。如先前在感染R.Rickettsii的兔子上饲喂microplusin沉默的蜱时获得的。这是第一份报告显示R.rickettsii感染通过肛门孔在弱视壁虱中，强调这条途径是评估其他靶标在病原体控制中的作用的有力工具。
