PDF(Pubmed)
Abstract：
Cancer is a significant health problem worldwide; consequently, new therapeutic alternatives are being investigated, including those found in the vegetable kingdom. Eugenol (Eug) has attracted attention for its therapeutic properties, especially in stomatology. The purpose of this study was to investigate the cytotoxicity of Eug, in vitro, on osteosarcoma (SAOS-2) and oropharyngeal squamous cancer (Detroit-562) cells, as well as its potential irritant effect in ovo at the level of the chorioallantoic membrane (CAM). The data obtained following a 72 h Eug treatment highlighted the reduction in cell viability up to 41% in SAOS-2 cells and up to 37% in Detroit-562 cells, respectively. The apoptotic-like effect of Eug was indicated by the changes in cell morphology and nuclear aspect; the increase in caspase-3/7, -8 and -9 activity; the elevated expression of Bax and Bad genes; and the increase in luminescence signal (indicating phosphatidylserine externalization) that preceded the increase in fluorescence signal (indicating the compromise of membrane integrity). Regarding the vascular effects, slight signs of coagulation and vascular lysis were observed, with an irritation score of 1.69 for Eug 1 mM. Based on these results, the efficiency of Eug in cancer treatment is yet to be clarified.
摘要：
癌症是全球范围内的重大健康问题；因此，正在研究新的治疗替代方案，包括在蔬菜王国发现的那些。丁香酚（Eug）因其治疗特性而备受关注，尤其是在口腔医学。本研究的目的是研究Eug的细胞毒性，在体外，骨肉瘤（SAOS-2）和口咽鳞癌（Detroit-562）细胞，以及其在绒毛尿囊膜（CAM）水平的卵中潜在的刺激作用。72小时Eug处理后获得的数据突出了SAOS-2细胞中细胞活力的降低高达41％，底特律-562细胞中细胞活力的降低高达37％。分别。Eug的凋亡样作用由细胞形态和核方面的变化指示;caspase-3/7、-8和-9活性的增加;Bax和Bad基因的表达升高;以及发光信号的增加(表明磷脂酰丝氨酸外化),其先于荧光信号的增加(表明膜完整性的损害)。关于血管效应，观察到轻微的凝血和血管溶解的迹象，Eug1mM的刺激评分为1.69。基于这些结果,Eug在癌症治疗中的有效性尚待阐明。
