PDF(Pubmed)
Abstract：
This study aims to activate the external urethral sphincter (EUS), which plays a critical role in micturition control, through optogenetics and to determine its potential contribution to the stabilization of sensitized micturition activity. The viral vector (AAV2/8-CMV-hChR2(H134R)-EGFP) is utilized to introduce light-gated ion channels (hChR2/H134R) into the EUS of wild-type C57BL/6 mice. Following the induction of sensitized micturition activity using weak acetic acid (0.1%) in anesthetized mice, optical stimulation of the EUS muscle tissue expressing channel rhodopsin is performed using a 473 nm laser light delivered through optical fibers, and the resulting changes in muscle activation and micturition activity are examined. Through EMG (electromyography) measurements, it is confirmed that optical stimulation electrically activates the EUS muscle in mice. Analysis of micturition activity using cystometry reveals a 70.58% decrease in the micturition period and a 70.27% decrease in the voiding volume due to sensitized voiding. However, with optical stimulation, the micturition period recovers to 101.49%, and the voiding volume recovered to 100.22%. Stimulation of the EUS using optogenetics can alleviate sensitized micturition activity and holds potential for application in conjunction with other micturition control methods.
摘要：
本研究旨在激活尿道外括约肌(EUS),在排尿控制中起着至关重要的作用，通过光遗传学，并确定其对敏化排尿活性稳定的潜在贡献。病毒载体(AAV2/8-CMV-hChR2(H134R)-EGFP)用于将光门控离子通道(hChR2/H134R)引入野生型C57BL/6小鼠的EUS中。在麻醉小鼠中使用弱乙酸（0.1％）诱导致敏排尿活动后，EUS肌肉组织表达通道视紫红质的光学刺激是使用通过光纤传递的473nm激光进行的，并检查肌肉激活和排尿活动的变化。通过EMG（肌电图）测量，证实了光学刺激电激活小鼠的EUS肌肉。使用膀胱测压法分析排尿活动显示，由于致敏排尿，排尿时间减少了70.58％，排尿量减少了70.27％。然而,通过光学刺激，排尿时间恢复到101.49%，排尿量恢复至100.22%。使用光遗传学刺激EUS可以减轻敏化的排尿活性，并具有与其他排尿控制方法联合应用的潜力。
