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Abstract：
Meibomian gland dysfunction (MGD) is a highly prevalent condition and the most common cause of evaporative dry eye disease. Studying the proteome of MGD can result in important advances in the management of the condition. Here, we collected tear film samples from treatment naïve patients with MGD (n = 10) and age-matched controls (n = 11) with Schirmer filtration paper. The samples were analyzed with label-free quantification nano liquid chromatography-tandem mass spectrometry. The proteins were considered differentially expressed if p < 0.05. A total of 88 proteins were significantly regulated. The largest change was observed in cystatin-SN, which was downregulated in MGD and correlated negatively with tear meniscus height. The downregulation of cystatin-SN was confirmed with targeted mass spectrometry by single reaction monitoring (SRM). Eighteen immunoglobulin components involved in B cell activation, phagocytosis, and complement activation were downregulated in MGD including Ig alpha-1 chain C region, immunoglobulin J chain, immunoglobulin heavy variable 3-15, and Ig mu chain C region. The changes in cystatin-SN and immunoglobulin chains are likely to result from the inflammatory changes related to tear film evaporation, and future studies may assess their association with the meibum quality.
摘要：
睑板腺功能障碍（MGD）是一种非常普遍的疾病，也是蒸发性干眼病的最常见原因。研究MGD的蛋白质组可以在疾病管理方面取得重要进展。这里,我们使用Schirmer滤纸从MGD初治患者(n=10)和年龄匹配的对照组(n=11)收集泪膜样本。样品采用无标记定量纳米液相色谱-串联质谱法进行分析。如果p<0.05,则认为蛋白质差异表达。共有88种蛋白质被显著调节。在胱抑素-SN中观察到最大的变化，在MGD中下调，与泪液半月板高度呈负相关。通过单反应监测(SRM)用靶向质谱确认胱抑素-SN的下调。参与B细胞活化的18种免疫球蛋白成分，吞噬作用,补体激活在MGD中下调，包括Igα-1链C区，免疫球蛋白J链,免疫球蛋白重变量3-15和Igmu链C区。胱抑素-SN和免疫球蛋白链的变化可能是由与泪膜蒸发相关的炎症变化引起的。未来的研究可能会评估它们与颜值质量的关联。
