PDF(Pubmed)
Abstract：
(1) Background: Sepsis is present in nearly 90% of critically ill patients with community-acquired pneumonia (CAP). This systematic review updates the information on studies that have assessed gene expression profiles in critically ill septic patients with CAP. (2) Methods: We searched for studies that satisfied the following criteria: (a) expression profile in critically ill patients with sepsis due to CAP, (b) presence of a control group, and (c) adult patients. Over-representation analysis was performed with clusterProfiler using the Hallmark and Reactome collections. (3) Results: A total of 4312 differentially expressed genes (DEGs) and sRNAs were included in the enrichment analysis. In the Hallmark collection, genes regulated by nuclear factor kappa B in response to tumor necrosis factor, genes upregulated by signal transducer and activator of transcription 5 in response to interleukin 2 stimulation, genes upregulated in response to interferon-gamma, genes defining the inflammatory response, a subgroup of genes regulated by MYC-version 1 (v1), and genes upregulated during transplant rejection were significantly enriched in critically ill septic patients with CAP. Moreover, 88 pathways were identified in the Reactome database. (4) Conclusions: This study summarizes the reported DEGs in critically ill septic patients with CAP and investigates their functional implications. The results highlight the complexity of immune responses during CAP.
摘要：
（1）背景：近90％的社区获得性肺炎（CAP）危重患者存在脓毒症。本系统综述更新了评估重症脓毒症患者CAP基因表达谱的研究信息。（2）方法：我们搜索满足以下标准的研究：（a）因CAP引起的脓毒症的危重患者的表达谱，(b)对照组的存在，（c）成人患者。使用ClusterProfiler使用Hallmark和Reactome集合进行过度表示分析。(3)结果:共4312个差异表达基因(DEGs)和sRNAs被包括在富集分析中。在Hallmark集合中，核因子κB调控的基因对肿瘤坏死因子的反应，响应于白细胞介素2刺激，由信号转导和转录激活因子5上调的基因，响应干扰素-γ的基因上调，定义炎症反应的基因，由MYC-版本1（v1）调节的一组基因，并且在移植排斥反应期间上调的基因在患有CAP的危重脓毒症患者中显著富集。此外，在Reactome数据库中鉴定了88条途径。（4）结论：本研究总结了重症脓毒症患者CAP的DEGs报告，并调查了其功能意义。结果突出了CAP期间免疫应答的复杂性。
