关键词: ERK-CREB-BDNF HPA axis Sargassum horneri corticosterone depression neurotransmitter

来  源:   DOI:10.3390/antiox12101841   PDF(Pubmed)

Abstract:
Sargassum horneri, a brown seaweed, is known for its various health benefits; however, there are no reports on its effects on depression. This study aimed to investigate the antidepressant effects of S. horneri ethanol extract (SHE) in mice injected with corticosterone (CORT) and to elucidate the underlying molecular mechanisms. Behavioral tests were conducted, and corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and CORT levels were measured. A fluorometric monoamine oxidase (MAO) enzyme inhibition assay was performed. Neurotransmitters like serotonin, dopamine, and norepinephrine levels were determined. Moreover, the ERK-CREB-BDNF signaling pathway in the prefrontal cortex and hippocampus was evaluated. Behavioral tests revealed that SHE has antidepressant effects by reducing immobility time and increasing time spent in open arms. Serum CRH, ACTH, and CORT levels decreased in the mice treated with SHE, as did the glucocorticoid-receptor expression in their brain tissues. SHE inhibited MAO-A and MAO-B activities. In addition, SHE increased levels of neurotransmitters. Furthermore, SHE activated the ERK-CREB-BDNF pathway in the prefrontal cortex and hippocampus. These findings suggest that SHE has antidepressant effects in CORT-injected mice, via the regulation of the hypothalamic-pituitary-adrenal axis and monoaminergic pathway, and through activation of the ERK-CREB-BDNF signaling pathway. Thus, our study suggests that SHE may act as a natural antidepressant.
摘要:
Sargassumhorneri,棕色的海藻,以其各种健康益处而闻名;然而,没有关于它对抑郁症的影响的报道。本研究旨在探讨霍氏链球菌乙醇提取物(SHE)对皮质酮(CORT)小鼠的抗抑郁作用,并阐明其潜在的分子机制。进行了行为测试,促肾上腺皮质激素释放激素(CRH),促肾上腺皮质激素(ACTH),并测量CORT水平。进行荧光单胺氧化酶(MAO)酶抑制测定。神经递质像血清素,多巴胺,并测定了去甲肾上腺素水平。此外,评估了前额叶皮质和海马中的ERK-CREB-BDNF信号通路。行为测试表明,SHE通过减少不动的时间和增加张开双臂的时间而具有抗抑郁作用。血清CRH,ACTH,用SHE治疗的小鼠的CORT水平下降,脑组织中糖皮质激素受体的表达也是如此。SHE抑制MAO-A和MAO-B活性。此外,SHE增加了神经递质的水平。此外,SHE激活前额叶皮质和海马中的ERK-CREB-BDNF通路。这些发现表明,SHE在注射CORT的小鼠中具有抗抑郁作用,通过调节下丘脑-垂体-肾上腺轴和单胺能途径,并通过激活ERK-CREB-BDNF信号通路。因此,我们的研究表明,SHE可能是一种天然的抗抑郁药.
公众号