PDF(Pubmed)
Abstract：
Selenium nanoparticles (SeNPs) are worthy of attention and development for nutritional supplementation due to their health benefits in both animals and humans with low toxicity, improved bioavailability, and controlled release, being greater than the Se inorganic and organic forms. Our previous study reported that Anoectochilus burmannicus extract (ABE)-synthesized SeNPs (ABE-SeNPs) exerted antioxidant and anti-inflammatory activities. Furthermore, ABE could stabilize and preserve the biological activities of SeNPs. To promote the ABE-SeNPs as supplementary and functional foods, it was necessary to carry out a safety assessment. Cytotoxicity testing showed that SeNPs and ABE-SeNPs were harmless with no killing effect on Caco2 (intestinal epithelial cells), MRC-5 (lung fibroblasts), HEK293 (kidney cells), LX-2 (hepatic stellate cells), and 3T3-L1 (adipocytes), and were not toxic to isolated human PBMCs and RBCs. Genotoxicity assessments found that SeNPs and ABE-SeNPs did not induce mutations in Salmonella typhimurium TA98 and TA100 (Ames test) as well as in Drosophila melanogaster (somatic mutation and recombination test). Noticeably, ABE-SeNPs inhibited mutation in TA98 and TA100 induced by AF-2, and in Drosophila induced by urethane, ethyl methanesulfonate, and mitomycin c, suggesting their anti-mutagenicity ability. This study provides data that support the safety and anti-genotoxicity properties of ABE-SeNPs for the further development of SeNPs-based food supplements.
摘要：
硒纳米颗粒（SeNPs）是值得关注和发展的营养补充，因为它们在动物和人类的健康益处，低毒性，提高生物利用度，和受控释放，大于Se无机和有机形式。我们先前的研究报道了金线莲提取物（ABE）合成的SeNPs（ABE-SeNPs）具有抗氧化和抗炎活性。此外，ABE可以稳定和保持SeNPs的生物活性。为了推广ABE-SeNPs作为辅助和功能食品，有必要进行安全评估。细胞毒性试验表明，SeNPs和ABE-SeNPs无害，对Caco2（肠上皮细胞）无杀伤作用，MRC-5（肺成纤维细胞），HEK293（肾细胞），LX-2（肝星状细胞），和3T3-L1（脂肪细胞），并且对分离的人PBMC和RBC无毒。遗传毒性评估发现，SeNPs和ABE-SeNPs不会在鼠伤寒沙门氏菌TA98和TA100（Ames测试）以及果蝇（体细胞突变和重组测试）中诱导突变。值得注意的是，ABE-SeNPs抑制由AF-2诱导的TA98和TA100以及由氨基甲酸酯诱导的果蝇中的突变，甲磺酸乙酯,和丝裂霉素C,表明了它们的抗诱变能力。这项研究提供了支持ABE-SeNPs的安全性和抗遗传毒性特性的数据，以进一步开发基于SeNPs的食品补充剂。
