Abstract：
Basic and clinical cancer research requires tumor models that consistently recapitulate the characteristics of prima tumors. As ex vivo 3D cultures of patient tumor cells, patient-derived tumor organoids possess the biological properties of primary tumors and are therefore excellent preclinical models for cancer research. Patient-derived organoids can be established using primary tumor tissues, peripheral blood, pleural fluid, ascites, and other samples containing tumor cells. Circulating tumor cells acquired by non-invasive sampling feature dynamic circulation and high heterogeneity. Circulating tumor cell-derived organoids are prospective tools for the dynamic monitoring of tumor mutation evolution profiles because they reflect the heterogeneity of the original tumors to a certain extent. This review discusses the advantages and applications of patient-derived organoids. Meanwhile, this work highlights the biological functions of circulating tumor cells, the latest advancement in research of circulating tumor cell-derived organoids, and potential application and challenges of this technology.
摘要：
基础和临床癌症研究需要能够始终如一地概括原始肿瘤特征的肿瘤模型。作为患者肿瘤细胞的离体3D培养物，患者来源的肿瘤类器官具有原发性肿瘤的生物学特性，因此是癌症研究的优秀临床前模型。可以使用原发性肿瘤组织建立患者来源的类器官，外周血,胸腔积液,腹水,和其他含有肿瘤细胞的样本。非侵入性采样获得的循环肿瘤细胞具有动态循环和高度异质性。循环肿瘤细胞来源的类器官是动态监测肿瘤突变演变谱的前瞻性工具，因为它们在一定程度上反映了原始肿瘤的异质性。本文综述了患者来源的类器官的优势和应用。同时,这项工作突出了循环肿瘤细胞的生物学功能，循环肿瘤细胞来源的类器官研究的最新进展，以及该技术的潜在应用和挑战。
