PDF(Pubmed)
Abstract：
Photosynthesis drives plant physiology, biomass accumulation, and yield. Photosynthetic efficiency, specifically the operating efficiency of PSII (Fq\'/Fm\'), is highly responsive to actual growth conditions, especially to fluctuating photosynthetic photon fluence rate (PPFR). Under field conditions, plants constantly balance energy uptake to optimize growth. The dynamic regulation complicates the quantification of cumulative photochemical energy uptake based on the intercepted solar energy, its transduction into biomass, and the identification of efficient breeding lines. Here, we show significant effects on biomass related to genetic variation in photosynthetic efficiency of 178 climbing bean (Phaseolus vulgaris L.) lines. Under fluctuating conditions, the Fq\'/Fm\' was monitored throughout the growing period using hand-held and automated chlorophyll fluorescence phenotyping. The seasonal response of Fq\'/Fm\' to PPFR (ResponseG:PPFR) achieved significant correlations with biomass and yield, ranging from 0.33 to 0.35 and from 0.22 to 0.31 in two glasshouse and three field trials, respectively. Phenomic yield prediction outperformed genomic predictions for new environments in four trials under different growing conditions. Investigating genetic control over photosynthesis, one single nucleotide polymorphism (Chr09_37766289_13052) on chromosome 9 was significantly associated with ResponseG:PPFR in proximity to a candidate gene controlling chloroplast thylakoid formation. In conclusion, photosynthetic screening facilitates and accelerates selection for high yield potential.
摘要：
光合作用驱动植物生理学，生物量积累和产量。光合效率,特别是光系统II的运行效率(Fq\'/Fm\')，对实际生长条件高度敏感，特别是波动的光合光子通量速率（PPFR）。在现场条件下,植物不断平衡能量吸收以优化生长。动态调节使基于截获的太阳能的累积光化学能量吸收的量化变得复杂。将其转化为生物量并鉴定高效育种系。这里,我们显示了对178个攀缘豆（PhaseolusvulgarisL.）品系光合效率遗传变异相关的生物量的显着影响。在波动的条件下，在整个生长期使用手持和自动叶绿素荧光表型分析监测Fq'/Fm'。在两个温室和三个田间试验中，Fq'/Fm'对PPFR（响应G：PPFR）的季节性响应与生物量和产量的显着相关性为0.33至0.35和0.22至0.31，分别。在相当不同的生长条件下，在四个试验中，对新环境的表型产量预测优于基因组预测。研究光合作用的遗传控制，9号染色体上的一个SNP（Chr09_37766289_13052）与ResponseG：PPFR显着相关，靠近控制叶绿体类囊体形成的候选基因。总之,光合筛选有助于并加速高产潜力的选择。
