Mice were given daily doses of whole inactivated influenza virus vaccine for periods of 14, 21, or 28 days; the control group received prime-boost immunization with a 28 days interval.
Our data show that the highest levels of cellular and humoral immune responses were induced by 28 days of extended antigen delivery, followed by 21, and 14 days of delivery, and prime-boost immunization. Moreover, prolonging vaccine delivery also improved the quality of the induced antibody response, as indicated by higher level of high avidity antibodies, a balanced IgG subclass profile, and a higher level of cross-reactive antibodies.
Our findings contribute to a better understanding of the immune response to influenza vaccination and have important implications for the design and development of future slow-release influenza vaccines.
给小鼠每日剂量的全灭活流感病毒疫苗,为期14、21或28天;对照组接受间隔28天的初免-加强免疫。
■我们的数据表明,延长28天的抗原递送可诱导最高水平的细胞和体液免疫反应,其次是21天和14天的交货时间,和强化免疫。此外,延长疫苗递送也提高了诱导的抗体反应的质量,高亲合力抗体水平较高,平衡的IgG亚类概况,和更高水平的交叉反应抗体。
■我们的发现有助于更好地了解流感疫苗接种的免疫反应,并对未来缓释流感疫苗的设计和开发具有重要意义。