Abstract：
Gossypol and the related terpenoids are stored in the pigment gland to protect cotton plants from biotic stresses, but little is known about the synthetic sites of these metabolites. Here, we showed that GoPGF, a key gene regulating gland formation, was expressed in gland cells and roots. The chromatin immunoprecipitation sequencing (ChIP-seq) analysis demonstrated that GoPGF targets GhJUB1 to regulate gland morphogenesis. RNA-sequencing (RNA-seq) showed high accumulation of gossypol biosynthetic genes in gland cells. Moreover, integrated analysis of the ChIP-seq and RNA-seq data revealed that GoPGF binds to the promoter of several gossypol biosynthetic genes. The cotton callus overexpressing GoPGF had dramatically increased the gossypol levels, indicating that GoPGF can directly activate the biosynthesis of gossypol. In addition, the gopgf mutant analysis revealed the existence of both GoPGF-dependent and -independent regulation of gossypol production in cotton roots. Our study revealed that the pigment glands are synthetic sites of gossypol in aerial parts of cotton and that GoPGF plays a dual role in regulating gland morphogenesis and gossypol biosynthesis. The study provides new insights for exploring the complex relationship between glands and the metabolites they store in cotton and other plant species.
摘要：
棉酚和相关萜类物质储存在色素腺中，以保护棉花植物免受生物胁迫，但是对这些代谢物的合成位点知之甚少。这里,我们发现GoPGF,调节腺体形成的关键基因，在腺细胞和根中表达。染色质免疫沉淀测序（ChIP-seq）分析表明，GoPGF靶向GhJUB1以调节腺体形态发生。RNA测序（RNA-seq）显示，腺细胞中棉酚生物合成基因的高度积累。此外，对ChIP-seq和RNA-seq数据的综合分析显示，GoPGF与几种棉酚生物合成基因的启动子结合。过表达GoPGF的棉花愈伤组织显著提高了棉酚的含量,说明GoPGF能直接激活棉酚的生物合成。此外,gopgf突变体分析揭示了棉花根中棉酚生产的GoPGF依赖性和非依赖性调节。我们的研究表明，色素腺是棉花地上部分棉酚的合成位点，GoPGF在调节腺体形态发生和棉酚生物合成中起双重作用。该研究为探索腺体与它们在棉花和其他植物物种中储存的代谢物之间的复杂关系提供了新的见解。
