UNASSIGNED: Individual ocular swabs (n = 168) were collected from cattle that had clinical signs of IBK during the summer of 2022 on farms in the Abay region of Kazakhstan. Eye lesion scores (1, 2, and 3) were assigned depending on the degree of ocular damage. Infectious bovine keratoconjunctivitis-associated organisms were detected using a multiplex real-time polymerase chain reaction assay. The Mor. bovoculi pilA gene was sequenced from Mor. bovoculi positive samples.
UNASSIGNED: Mycoplasma bovis and BHV-1 were not detected in any of the collected samples. Mycoplasma bovoculi was identified in the majority of samples overall, usually in mixed infection with Moraxella spp. Moraxella bovoculi was detected in 76.2% of animals and predominated in animals with eye lesion scores 2 and 3. Mycoplasma bovoculi was detected only in association with Mor. bovis and/or Mor. bovoculi in animals with eye lesion scores 2 and 3. Moraxella bovis was found in 57.7% of animals and was always identified in association with another organism. Sequencing of the pilA gene in 96 samples from Mor. bovoculi positive samples identified five PilA groups. The majority belonged to PilA group A. However, three new PilA groups were identified and designated PilA groups N, O, and P.
UNASSIGNED: The results indicate a high prevalence of Myc. bovoculi and Mor. bovoculi in eyes of cattle with IBK on livestock farms in Eastern Kazakhstan. Additional novel Mor. bovoculi PilA groups were identified.
■从2022年夏季在哈萨克斯坦阿拜地区的农场中收集了具有IBK临床症状的牛的单个眼拭子(n=168)。根据眼部损伤的程度分配眼部病变评分(1、2和3)。使用多重实时聚合酶链反应测定法检测传染性牛角膜结膜炎相关生物。Mor.对Mor的bovoculipilA基因进行了测序。bovoculi阳性样本。
■在任何收集的样品中均未检测到牛支原体和BHV-1。在总体上大多数样品中都鉴定出了猪粪支原体,通常与莫拉氏菌属混合感染。在76.2%的动物中检测到了Bovoculi莫拉氏菌,在眼部病变评分为2和3的动物中占主导地位。仅在与Mor相关的情况下检测到了猪粪支原体。bovis和/或Mor.眼睛病变得分为2和3的动物的bovoculi。在57.7%的动物中发现了牛莫拉氏菌,并且总是与另一种生物相关联。来自Mor的96个样品中的pilA基因的测序。bovoculi阳性样本确定了五个PilA组。大多数属于PilA组。然而,确定了三个新的PilA组,并将其指定为PilA组N,O,和P.
■结果表明Myc的患病率很高。Bovoculi和Mor.bovoculi在哈萨克斯坦东部的牲畜农场与IBK的牛眼中。其他小说Mor确定了bovoculiPilA组。