Abstract：
Hepatocellular carcinoma (HCC) is the most frequent primary malignancy of the liver. The aim of this study is to evaluate the comparative in silico and in vivo ameliorative potential of the ethanolic extract of Curcuma longa (EECL) in male and female Wistar rats administered N-nitrosodiethylamine-induced hepatocellular carcinoma. The MAPK compound was obtained from a protein data bank (PDB ID: 7AUV) for molecular docking. One hundred and twenty Wistar rats, were randomly selected into twelve groups (n = 5): Group A received regular diets as a basal control; groups B to G were administered 100 mg/kg NDEA twice in two weeks; while groups C to E received 200 mg/kg, 400 mg/kg, and 600 mg/kg of EECL; group F was treated with 200 mg/kg pure curcumin; and group G received 100 mg/kg Sylibon-140. Group H received only 200 mg/kg pure curcumin, and group I received 200 mg/kg of dimethylsulfoxide (DMSO). Groups J, K, and L received 200 mg/kg, 400 mg/kg and 600 mg/kg of EECL. MAPK and AFP mRNA in Wistar rats administered NDEA were upregulated as compared to EECL groups. In conclusion, the in silico and in vitro study validates the mitigating role of ethanolic extract of Curcuma longa and pure curcumin.
摘要：
肝细胞癌（HCC）是肝脏最常见的原发性恶性肿瘤。这项研究的目的是评估在雄性和雌性Wistar大鼠中服用N-亚硝基二乙胺诱导的肝细胞癌的姜黄乙醇提取物（EECL）的比较和体内改善潜力。MAPK化合物从用于分子对接的蛋白质数据库(PDBID:7AUV)获得。一百二十只Wistar老鼠，随机分为12组（n=5）：A组接受常规饮食作为基础对照；B至G组在两周内两次给予100mg/kgNDEA；而C至E组接受200mg/kg，400mg/kg,和600mg/kg的EECL；F组用200mg/kg纯姜黄素治疗；G组接受100mg/kg的Sylibon-140。H组仅接受200mg/kg纯姜黄素，和组I接受200mg/kg的二甲基亚砜(DMSO)。J组,K,L接受200mg/kg，400mg/kg和600mg/kg的EECL。与EECL组相比，给予NDEA的Wistar大鼠中的MAPK和AFPmRNA上调。总之,计算机模拟和体外研究验证了姜黄的乙醇提取物和纯姜黄素的缓解作用。
