PDF(Pubmed)
Abstract：
Effective preventive measures against postoperative cognitive dysfunction in older adults are urgently needed. In this study, we investigated the effect of electroacupuncture (EA) on anesthesia and surgery-induced cognitive decline in aged rats by RNA-seq analysis, behavioral testing, Golgi-Cox staining, dendritic spine analysis, immunofluorescence assay and western blot analysis. EA ameliorated anesthesia and surgery induced-cognitive decline. RNA-seq analysis identified numerous differentially-expressed genes, including 353 upregulated genes and 563 downregulated genes, after pretreatment with EA in aged rats with postoperative cognitive dysfunction. To examine the role of CREB in EA, we injected adeno-associated virus (AAV) into the CA1 region of the hippocampus bilaterally into the aged rats to downregulate the transcription factor. EA improved synaptic plasticity, structurally and functionally, by activating the MAPK/ERK/CREB signaling pathway in aged rats. Together, our findings suggest that EA protects against anesthesia and surgery-induced cognitive decline in aged rats by activating the MAPK/ERK/CREB signaling pathway and enhancing hippocampal synaptic plasticity.
摘要：
迫切需要针对老年人术后认知功能障碍的有效预防措施。在这项研究中,我们通过RNA-seq分析研究了电针（EA）对老年大鼠麻醉和手术引起的认知功能下降的影响，行为测试，高尔基-考克斯染色,树突棘分析,免疫荧光分析和蛋白质印迹分析。EA改善麻醉和手术引起的认知功能下降。RNA-seq分析确定了许多差异表达的基因，包括353个上调基因和563个下调基因，EA预处理后老年大鼠术后认知功能障碍。为了检查CREB在EA中的作用，我们向老龄大鼠双侧海马CA1区注射腺相关病毒(AAV),下调转录因子.EA改善突触可塑性，结构和功能，通过激活老年大鼠MAPK/ERK/CREB信号通路。一起,我们的研究结果表明,电针通过激活MAPK/ERK/CREB信号通路和增强海马突触可塑性,防止麻醉和手术引起的老年大鼠认知功能下降.
