Abstract：
DNA methylation as intensively studied epigenetic regulatory mechanism exerts pleiotropic effects on dental-derived mesenchymal stem cells (DMSCs). DMSCs have self-renewal and multidifferentiation potential. Here, this review aims at summarizing the research status about application of DMSCs in tissue engineering and clarifying the roles of DNA methylation in influencing the functions of DMSCs, with expectation of paving the way for its in-depth exploration in tissue engineering.
The current research status about influence of DNA methylation in DMSCs was acquired by MEDLINE (through PubMed) and Web of Science using the keywords \'DNA methylation\', \'dental-derived mesenchymal stem cells\', \'dental pulp stem cells\', \'periodontal ligament stem cells\', \'dental follicle stem cells\', \'stem cells from the apical papilla\', \'stem cells from human exfoliated deciduous teeth\', and \'gingival-derived mesenchymal stem cells\'.
This review indicates DNA methylation affects DMSCs\' differentiation and function through inhibiting or enhancing the expression of specific gene resulted by DNA methylation-related genes or relevant inhibitors.
DNA methylation can influence DMSCs in aspects of osteogenesis, adipogenesis, immunomodulatory function, and so on. Yet, the present studies about DNA methylation in DMSCs commonly focus on dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs). Little has been reported for other DMSCs.
The current research status about influence of DNA methylation in DMSCs was acquired by MEDLINE (through PubMed) and Web of Science using the keywords \'DNA methylation\', \'dental-derived mesenchymal stem cells\', \'dental pulp stem cells\', \'periodontal ligament stem cells\', \'dental follicle stem cells\', \'stem cells from the apical papilla\', \'stem cells from human exfoliated deciduous teeth\', and \'gingival-derived mesenchymal stem cells\'.
This review indicates DNA methylation affects DMSCs\' differentiation and function through inhibiting or enhancing the expression of specific gene resulted by DNA methylation-related genes or relevant inhibitors.
DNA methylation can influence DMSCs in aspects of osteogenesis, adipogenesis, immunomodulatory function, and so on. Yet, the present studies about DNA methylation in DMSCs commonly focus on dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs). Little has been reported for other DMSCs.
摘要：
目的：DNA甲基化作为对表观遗传调控机制的深入研究，对牙源性间充质干细胞（DMSCs）具有多效性作用。DMSCs具有自我更新和多分化潜能。这里,综述了DMSCs在组织工程中的应用研究现状,阐明DNA甲基化对DMSCs功能的影响,以期为其在组织工程方面的深入探索铺平道路。
方法：MEDLINE（通过PubMed）和WebofScience使用关键字“DNA甲基化”获得了有关DMSCs中DNA甲基化影响的当前研究状况，牙源性间充质干细胞，“牙髓干细胞”，“牙周膜干细胞”，牙囊干细胞，“根尖乳头的干细胞”，“来自人类脱落乳牙的干细胞”，和牙龈间充质干细胞。
结果：本综述表明DNA甲基化通过抑制或增强DNA甲基化相关基因或相关抑制剂导致的特定基因的表达来影响DMSCs的分化和功能。
结论：DNA甲基化可影响DMSCs成骨,脂肪生成,免疫调节功能，等等。然而，目前关于DMSCsDNA甲基化的研究主要集中在牙髓干细胞（DPSCs）和牙周膜干细胞（PDLSCs）。对于其他DMSC几乎没有报道。
方法：MEDLINE（通过PubMed）和WebofScience使用关键字“DNA甲基化”获得了有关DMSCs中DNA甲基化影响的当前研究状况，牙源性间充质干细胞，“牙髓干细胞”，“牙周膜干细胞”，牙囊干细胞，“根尖乳头的干细胞”，“来自人类脱落乳牙的干细胞”，和牙龈间充质干细胞。
结果：本综述表明DNA甲基化通过抑制或增强DNA甲基化相关基因或相关抑制剂导致的特定基因的表达来影响DMSCs的分化和功能。
结论：DNA甲基化可影响DMSCs成骨,脂肪生成,免疫调节功能，等等。然而，目前关于DMSCsDNA甲基化的研究主要集中在牙髓干细胞（DPSCs）和牙周膜干细胞（PDLSCs）。对于其他DMSC几乎没有报道。
