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Abstract：
Background In the periodontal regenerative procedure, the membrane used should possess good mechanical stability with suitable resorption time to allow restoration of the lost periodontium. Amniotic membrane (AM) has regenerative potential as a scaffold or barrier membrane due to its various beneficial properties. However, its degradation rate is not clearly reported. Methodology This study aimed to evaluate the resorption capacity of AM and its surface architecture after being subjected to hydrolytic degradation analysis in phosphate buffer solution (PBS). AM was cut into sizes of 10 × 10 mm2 for three replicates. The membranes were weighed before and at different time intervals (days 7, 14, 21, and 28) after immersion in PBS. The degradation rate was determined by the percentage of mean weight loss from the initial weight at different time intervals. The AM surface profile was observed under scanning electron microscopy (SEM) before and after 28 days of immersion. Results The result shows a 92% loss of weight over 28 days with the highest attained in the first seven days (67%), followed by 7%, 17%, and 1% after days 14, 21, and 28, respectively. SEM of the AM surface before the degradation test showed a polygonal shape forming a well-arranged mosaic pattern covered with microvilli. At day 28, the remaining AM appears as porous surface architecture, irregularly arranged fibers, and no microvilli seen. Conclusions This study demonstrated that over four weeks of degradation analysis, AM was not entirely degraded but had lost some of the microstructure. The biodegradability of AM should be further evaluated to elucidate its stability within adequate time parallel with the tissue healing process in periodontal tissue regeneration.
摘要：
背景技术在牙周再生过程中,使用的膜应具有良好的机械稳定性，并具有合适的吸收时间，以恢复丢失的牙周组织。羊膜（AM）由于其各种有益特性而具有作为支架或屏障膜的再生潜力。然而,其降解率没有明确报告。方法本研究旨在评估在磷酸盐缓冲溶液（PBS）中进行水解降解分析后，AM的再吸收能力及其表面结构。将AM切成10×10mm2的大小,重复三次。在浸入PBS中之前和之后的不同时间间隔(第7、14、21和28天)对膜称重。降解速率通过在不同时间间隔下从初始重量的平均重量损失的百分比来确定。在浸没28天之前和之后，在扫描电子显微镜（SEM）下观察AM表面轮廓。结果结果显示，在28天内体重减轻了92％，在前7天达到了最高（67％），其次是7%,17%,在第14、21和28天后分别为1%。在降解测试之前，AM表面的SEM显示出多边形形状，形成了覆盖有微绒毛的排列良好的马赛克图案。在第28天，剩余的AM表现为多孔表面结构，不规则排列的纤维，没有看到微绒毛。结论本研究表明，经过四周的降解分析，AM没有完全降解，但失去了一些微观结构。应进一步评估AM的生物降解性，以在与牙周组织再生中的组织愈合过程平行的足够时间内阐明其稳定性。
