Abstract：
It aimed to explore the correlation of Glu504Lys locus mutation of aldehyde dehydrogenase-2 (ALDH2) with coronary heart disease (CHD) based on gold magnetic nanoparticles (GMNPs) chromatography and amplification refractory mutation system-PCR (ARMS-PCR). 120 CHD patients admitted to the cardiovascular Department of Wenling First People\'s Hospital affiliated to Wenzhou Medical University from December 2020 to December 2021 were selected as Case group and 80 non-CHD patients admitted during the same period were selected as Ctrl group. The venous blood and indexes of Total Cholesterol (TC), Triglyceride (TG), Low Density Lipoprotein Cholesterol (LDL-C), High Density Lipoprotein Cholesterol (HDL-C), and Fasting Blood Glucose (FBS) were collected. The ARMS-PCR GMNPs chromatography based on ARMS-PCR and immunochromatography assay was adopted to detect gene polymorphism of ALDH2. Correlation between ALDH2 gene polymorphism and risk factors of CHD was analyzed via logistic regression. In contrast to Ctrl group, the genotypes of GG, GA, and AA in Case group were evidently different (P < 0.05), and the frequency of A allelic gene was obviously increased (P < 0.05). Under the dominant model, frequency of GA + AA genotype in Case group was remarkably higher in contrast to Ctrl group (P < 0.05). Under the recessive model, there was no obvious difference in genotype frequency between two groups. In contrast to Ctrl group, TC, LDL-C, and FBS in Case group were notably increased (P < 0.05), while HDL-C was notably decreased (P < 0.05). The distribution frequency of abnormal LDL-C, HDL-C, and FBS in Case group was notably higher in contrast to Ctrl group (P < 0.05). LDL-C and FBS had no obvious effect on the genotypes and frequency distribution of alleles in CHD patients. However, the frequency distribution of genotypes of GA and AA and A allelic gene in patients with abnormal HDL-C was notably lower in contrast to those with normal HDL-C (P < 0.05). Logistic regression analysis showed that abnormal HDC-C with A allelic gene were independent risk factors for CHD (P = 0.001, OR = 1.934). The gene polymorphism of Glu504Lys locus of ALDH2 was closely related to the pathogenesis of CHD, A allelic gene may be a susceptibility gene for CHD, and patients with abnormal HDC-C and carried A allelic gene had relatively higher incidence of CHD.
摘要：
目的基于金磁性纳米粒子(GMNPs)色谱和扩增难突变系统-PCR(ARMS-PCR)探讨醛脱氢酶-2(ALDH2)Glu504Lys位点突变与冠心病(CHD)的相关性。选取温州医科大学附属温岭市第一人民医院心血管内科2020年12月至2021年12月收治的冠心病患者120例为病例组,选取同期收治的非冠心病患者80例为对照组。静脉血和总胆固醇(TC)指标,甘油三酯（TG），低密度脂蛋白胆固醇（LDL-C），高密度脂蛋白胆固醇(HDL-C),收集空腹血糖(FBS)。采用基于ARMS-PCR和免疫层析法的ARMS-PCRGMNPs色谱检测ALDH2基因多态性。采用logistic回归分析ALDH2基因多态性与冠心病危险因素的相关性。与Ctrl组相比，GG的基因型，GA,病例组与AA比较差异有统计学意义(P<0.05),A等位基因频率明显增加(P<0.05)。在主导模式下，病例组GA+AA基因型频率明显高于Ctrl组(P<0.05)。在隐性模式下，两组基因型频率无明显差异。与Ctrl组相比，TC,LDL-C,病例组FBS明显升高(P<0.05),HDL-C显著降低(P<0.05)。LDL-C异常分布频率,HDL-C,Case组FBS明显高于Ctrl组(P<0.05)。LDL-C和FBS对CHD患者等位基因的基因型和频率分布无明显影响。然而,HDL-C异常患者的GA，AA和A等位基因基因型的频率分布明显低于HDL-C正常患者（P<0.05）。Logistic回归分析显示HDC-C等位基因异常是冠心病的独立危险因素(P=0.001,OR=1.934)。ALDH2的Glu504Lys位点的基因多态性与冠心病的发病密切相关,等位基因可能是CHD的易感基因，HDC-C异常且携带A等位基因的患者的CHD发生率相对较高。
