PDF(Pubmed)
Abstract：
BACKGROUND: Oral submucous fibrosis (OSMF) is a habit related potentially malignant disorder seen mainly in South Asian people. The malignancy arising from OSMF has been regarded as low grade with better outcome. The present study was orchestrated to histochemically analyze collagen and elastic fibres in OSMF without dysplasia, OSMF with dysplasia and OSMF turning malignant.
METHODS: 100 cases (80 cases and 20 healthy controls) were included after obtaining clearance from ethical committee. All cases were subjected to Van Gieson staining for collagen and a novel simple method for elastic fibres (Orcein staining). Data were analyzed using SPSS software.
RESULTS: Controls showed haphazard arrangement of collagen and elastic fibres. The collagen bundles were parallelly arranged in OSMF without dysplasia and OSMF with dysplasia; the collagen was however haphazard in cases of OSMF turning malignant. As with collagen, elastic fibres were also haphazardly arranged in the control group; in contrast, the elastic fibres were predominantly arranged in a criss-cross pattern in the other study groups. The difference in orientation and density among the groups was statistically significant.
CONCLUSIONS: With advancement of stage there is increased collagenization of OSMF, as the condition acquires dysplastic changes and turns malignant, microenvironment alters resulting in increased activity of collagenases. However, the arrangement of more resistant elastic fibres depicts the better outcome, once OSMF shows malignant transformation, limiting locoregional and distant spread.
METHODS: 100 cases (80 cases and 20 healthy controls) were included after obtaining clearance from ethical committee. All cases were subjected to Van Gieson staining for collagen and a novel simple method for elastic fibres (Orcein staining). Data were analyzed using SPSS software.
RESULTS: Controls showed haphazard arrangement of collagen and elastic fibres. The collagen bundles were parallelly arranged in OSMF without dysplasia and OSMF with dysplasia; the collagen was however haphazard in cases of OSMF turning malignant. As with collagen, elastic fibres were also haphazardly arranged in the control group; in contrast, the elastic fibres were predominantly arranged in a criss-cross pattern in the other study groups. The difference in orientation and density among the groups was statistically significant.
CONCLUSIONS: With advancement of stage there is increased collagenization of OSMF, as the condition acquires dysplastic changes and turns malignant, microenvironment alters resulting in increased activity of collagenases. However, the arrangement of more resistant elastic fibres depicts the better outcome, once OSMF shows malignant transformation, limiting locoregional and distant spread.
摘要：
背景：口腔粘膜下纤维化（OSMF）是一种与习惯相关的潜在恶性疾病，主要见于南亚人群。由OSMF引起的恶性肿瘤被认为是低等级的，结果更好。本研究旨在组织化学分析OSMF中的胶原蛋白和弹性纤维，而没有发育异常，OSMF伴发育不良和OSMF恶性。
方法：获得伦理委员会批准后纳入100例（80例和20例健康对照）。所有病例均接受了VanGieson胶原蛋白染色和一种新颖的弹性纤维简单方法（Orcein染色）。数据采用SPSS软件进行分析。
结果：对照显示胶原蛋白和弹性纤维的随意排列。胶原蛋白束平行排列在无发育不良的OSMF和有发育不良的OSMF中；然而，在OSMF变成恶性的情况下，胶原蛋白是偶然的。和胶原蛋白一样，弹性纤维在对照组中也随意排列；相比之下，在其他研究组中,弹性纤维主要呈交叉排列.各组之间的取向和密度差异具有统计学意义。
结论：随着阶段的进展，OSMF的胶原化增加，随着病情发生发育不良并变成恶性，微环境改变导致胶原酶活性增加。然而,更耐久的弹性纤维的排列描述了更好的结果，一旦OSMF显示出恶性转化，限制局部和远距离传播。
方法：获得伦理委员会批准后纳入100例（80例和20例健康对照）。所有病例均接受了VanGieson胶原蛋白染色和一种新颖的弹性纤维简单方法（Orcein染色）。数据采用SPSS软件进行分析。
结果：对照显示胶原蛋白和弹性纤维的随意排列。胶原蛋白束平行排列在无发育不良的OSMF和有发育不良的OSMF中；然而，在OSMF变成恶性的情况下，胶原蛋白是偶然的。和胶原蛋白一样，弹性纤维在对照组中也随意排列；相比之下，在其他研究组中,弹性纤维主要呈交叉排列.各组之间的取向和密度差异具有统计学意义。
结论：随着阶段的进展，OSMF的胶原化增加，随着病情发生发育不良并变成恶性，微环境改变导致胶原酶活性增加。然而,更耐久的弹性纤维的排列描述了更好的结果，一旦OSMF显示出恶性转化，限制局部和远距离传播。
