Abstract：
Interstitial Cystitis (IC) is a chronic inflammatory disease that lacks effective treatment. The present study aimed to investigate the potential of aqueous ethanol extract of Cuminum cyminum (AEECC) on oxidative stress, inflammation and overactivity of urinary bladder induced by cyclophosphamide (CYP). Female Sprague-Dawley rats received intraperitoneal administration of cyclophosphamide (150 mg/kg, i. p. 1st , 4th , and 7th days). To investigate the urothelial damage, the bladder weight, nociception behavior, and Evans blue dye extravasation method was used. The antioxidants CAT, GPX and NO were measured. ELISA determined the IL-6 and TNF-α levels. The spasmolytic effect of AEECC was investigated on isolated bladder strips and its mechanisms were determined. The enhanced nociception behavior, bladder weight, vascular permeability, edema, hemorrhage, nitric oxide, IL-6 and TNF-α levels by CYP administration were significantly reduced by AEECC (250 and 500 mg/kg). A significant increase in serum antioxidant system such as CAT and GPx was also observed in AEECC-treated rats. The AEECC (3 mg/ml) significantly reduced urinary bladder tone in the strips pre-contracted with carbachol in both control and CYP-treated rats. This relaxation was demolished by atropine, nifedipine, glibenclamide, and indomethacin but not with propranolol. The plant extract showed the presence of antioxidant and anti-inflammatory phytochemicals. These results suggest that Cuminum cyminum offers uroprotective activity and can ameliorate CYP-induced bladder toxicity by modulating antioxidant parameters, pro-inflammatory cytokine levels and bladder smooth muscle overactivity. The in silico binding interactions of antioxidant 2I3Y and anti-inflammatory protein 1TNF with various ligands from Cuminum cyminum seeds revealed potential bioactive compounds with promising antioxidant and anti-inflammatory properties, providing valuable insights for drug development and nutraceutical research.
摘要：
本研究旨在研究乌贼水乙醇提取物(AEECC)对氧化应激的影响,环磷酰胺（CYP）引起的膀胱炎症和过度活跃。增强的伤害感受行为，膀胱重量,血管通透性,水肿,出血,一氧化氮,AEECC（250和500mg/kg）显着降低了CYP给药的IL-6和TNF-α水平。在AEECC处理的大鼠中还观察到血清抗氧化系统如CAT和GPx的显著增加。在对照和CYP处理的大鼠中，AEECC（3mg/ml）均显着降低了用卡巴胆碱预收缩的条带中的膀胱张力。这种放松被阿托品摧毁了，硝苯地平,格列本脲,和吲哚美辛，但没有普萘洛尔。植物提取物显示存在抗氧化剂和抗炎植物化学物质。这些结果表明，CuminumCuminum提供了泌尿保护活性，并可以通过调节抗氧化剂参数来改善CYP诱导的膀胱毒性。促炎细胞因子水平和膀胱平滑肌过度活跃。抗氧化剂2I3Y和抗炎蛋白1TNF与来自CuminumCuminumCuminumseeds的各种配体的计算机结合相互作用揭示了具有有希望的抗氧化和抗炎特性的潜在生物活性化合物，为药物开发和营养研究提供有价值的见解。
