PDF(Pubmed)
Abstract：
BACKGROUND: Oral cancer is the major cause of mortality and morbidity worldwide. There are many factors that influence the tumor microenvironment that promotes tumorigenesis. Hypoxia is one of the factors that affects the process of angiogenesis by inducing proangiogenic factors to maintain the blood supply which in turn enhances the aggressiveness of the tumor and prognosis of solid tumors such as oral squamous cell carcinoma.
OBJECTIVE: The aim of the study was to compare the expression of hypoxia-inducible factor 1α (HIF-1α) and hypoxia-inducible factor 2α (HIF-2α) in various histological grades of oral squamous cell carcinoma immunohistochemically.
METHODS: Immunohistochemical evaluation of HIF-1α and HIF-2α was done in 90 samples of oral squamous cell carcinoma which were graded histologically into 30 samples each of well, moderately and poorly differentiated squamous cell carcinoma. Statistical evaluation: Statistical analysis was done to study the prognostic significance of the biomarkers.
RESULTS: All the cases showed positivity for expression of HIF-1α and HIF-2α. The number of positive staining in both markers reduced as the tumor severity increased from well to poorly differentiated. The expression of MIL of HIF-2α was higher than HIF 1α and HIF 2α expression was mostly seen in cytoplasmic in well-differentiated and nuclear in both moderately and poorly differentiated OSCC suggestive that HIF-2α is a more specific marker to hypoxia.
CONCLUSIONS: Hypoxia is an essential factor that triggers other angiogenic switch and inflammatory factors which facilitates the process of tumorigenesis. This is also important for predicting the treatment outcome and prognosis of the patients. HIF-2α is a more sensitive marker that appears to be correlated and could perhaps serve as a good surrogate marker of hypoxia.
OBJECTIVE: The aim of the study was to compare the expression of hypoxia-inducible factor 1α (HIF-1α) and hypoxia-inducible factor 2α (HIF-2α) in various histological grades of oral squamous cell carcinoma immunohistochemically.
METHODS: Immunohistochemical evaluation of HIF-1α and HIF-2α was done in 90 samples of oral squamous cell carcinoma which were graded histologically into 30 samples each of well, moderately and poorly differentiated squamous cell carcinoma. Statistical evaluation: Statistical analysis was done to study the prognostic significance of the biomarkers.
RESULTS: All the cases showed positivity for expression of HIF-1α and HIF-2α. The number of positive staining in both markers reduced as the tumor severity increased from well to poorly differentiated. The expression of MIL of HIF-2α was higher than HIF 1α and HIF 2α expression was mostly seen in cytoplasmic in well-differentiated and nuclear in both moderately and poorly differentiated OSCC suggestive that HIF-2α is a more specific marker to hypoxia.
CONCLUSIONS: Hypoxia is an essential factor that triggers other angiogenic switch and inflammatory factors which facilitates the process of tumorigenesis. This is also important for predicting the treatment outcome and prognosis of the patients. HIF-2α is a more sensitive marker that appears to be correlated and could perhaps serve as a good surrogate marker of hypoxia.
摘要：
背景：口腔癌是全球死亡率和发病率的主要原因。有许多因素影响促进肿瘤发生的肿瘤微环境。缺氧是通过诱导促血管生成因子来维持血液供应而影响血管生成过程的因素之一,这反过来又增强了肿瘤的侵袭性和实体瘤如口腔鳞状细胞癌的预后。
目的：本研究的目的是比较缺氧诱导因子1α（HIF-1α）和缺氧诱导因子2α（HIF-2α）在口腔鳞状细胞癌不同组织学分级中的表达。
方法：对90个口腔鳞状细胞癌样本进行HIF-1α和HIF-2α的免疫组化评价，将其组织学分级为30个样本，中度和低分化鳞状细胞癌。统计评价:进行统计分析以研究生物标志物的预后意义。
结果：所有病例均显示HIF-1α和HIF-2α阳性表达。随着肿瘤严重程度从分化良好增加到分化差,两种标志物中的阳性染色数量减少。HIF-2α的MIL表达高于HIF1α，HIF2α的表达主要见于高分化的细胞质中，而中分化和低分化的OSCC的细胞核中，提示HIF-2α是缺氧的更特异性标志物。
结论：缺氧是引发其他血管生成转换和促进肿瘤发生过程的炎症因子的重要因素。这对于预测患者的治疗结果和预后也很重要。HIF-2α是一种更敏感的标志物，似乎是相关的，可能是缺氧的良好替代标志物。
目的：本研究的目的是比较缺氧诱导因子1α（HIF-1α）和缺氧诱导因子2α（HIF-2α）在口腔鳞状细胞癌不同组织学分级中的表达。
方法：对90个口腔鳞状细胞癌样本进行HIF-1α和HIF-2α的免疫组化评价，将其组织学分级为30个样本，中度和低分化鳞状细胞癌。统计评价:进行统计分析以研究生物标志物的预后意义。
结果：所有病例均显示HIF-1α和HIF-2α阳性表达。随着肿瘤严重程度从分化良好增加到分化差,两种标志物中的阳性染色数量减少。HIF-2α的MIL表达高于HIF1α，HIF2α的表达主要见于高分化的细胞质中，而中分化和低分化的OSCC的细胞核中，提示HIF-2α是缺氧的更特异性标志物。
结论：缺氧是引发其他血管生成转换和促进肿瘤发生过程的炎症因子的重要因素。这对于预测患者的治疗结果和预后也很重要。HIF-2α是一种更敏感的标志物，似乎是相关的，可能是缺氧的良好替代标志物。
