PDF(Pubmed)
Abstract：
UNASSIGNED: Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: COVID-19 convalescents who received antibiotics during hospitalization (I), COVID-19 convalescents without antibiotic therapy (II) and healthy individuals (III).
UNASSIGNED: Dental examination was conducted, and oral health status was evaluated using selected dental indexes. Clinical samples (saliva, dorsal swabs, supragingival and subgingival plaque) were collected and used for metagenomic library to the next-generation sequencing (NGS) preparation.
UNASSIGNED: Each of the clinical materials in particular groups of patients showed a statistically significant and quantitatively different bacterial composition. Patients from group I showed significantly worse oral health, reflected by higher average values of dental indexes and also a higher percentage of Veillonella, Tannerella, Capnocytophaga and Selenomonas genera in comparison to other groups. Additionally, a statistically significant decrease in the amount of Akkermansia type in both groups with COVID-19 was observed for all materials.
UNASSIGNED: The primary factor affecting the composition of oral microbiota was not the SARS-CoV-2 infection itself, but the use of antibiotic therapy. The increased percentage of pro-inflammatory pathogens observed in COVID-19 patients underscores the importance of preventing periodontal disease and improving oral hygiene in the future.
UNASSIGNED: Dental examination was conducted, and oral health status was evaluated using selected dental indexes. Clinical samples (saliva, dorsal swabs, supragingival and subgingival plaque) were collected and used for metagenomic library to the next-generation sequencing (NGS) preparation.
UNASSIGNED: Each of the clinical materials in particular groups of patients showed a statistically significant and quantitatively different bacterial composition. Patients from group I showed significantly worse oral health, reflected by higher average values of dental indexes and also a higher percentage of Veillonella, Tannerella, Capnocytophaga and Selenomonas genera in comparison to other groups. Additionally, a statistically significant decrease in the amount of Akkermansia type in both groups with COVID-19 was observed for all materials.
UNASSIGNED: The primary factor affecting the composition of oral microbiota was not the SARS-CoV-2 infection itself, but the use of antibiotic therapy. The increased percentage of pro-inflammatory pathogens observed in COVID-19 patients underscores the importance of preventing periodontal disease and improving oral hygiene in the future.
摘要：
口腔卫生不良以及牙周炎的发病率和严重程度增加可能会加剧SARS-CoV-2感染。目的是评估60名参与者的口腔微生物群，这些参与者分为两组：住院期间接受抗生素的COVID-19疗养者（I），没有抗生素治疗的COVID-19疗养者(II)和健康个体(III)。
■进行牙科检查，并使用选定的牙科指标评估口腔健康状况。临床样本（唾液，背侧拭子,收集牙龈上菌斑和牙龈下菌斑），并用于宏基因组文库以进行下一代测序（NGS）制备。
■在特定患者组中的每种临床材料显示出统计学上显著的和数量上不同的细菌组成。I组患者口腔健康状况明显恶化，反映在较高的牙科指数平均值和较高的Veillonella百分比，制革菌,与其他组相比，Capnocytophaga和硒单胞菌属。此外,在所有材料中，两组COVID-19患者的Akkermansia型数量均有统计学意义的下降。
■影响口腔微生物群组成的主要因素不是SARS-CoV-2感染本身，而是使用抗生素治疗。在COVID-19患者中观察到的促炎病原体百分比增加强调了未来预防牙周病和改善口腔卫生的重要性。
■进行牙科检查，并使用选定的牙科指标评估口腔健康状况。临床样本（唾液，背侧拭子,收集牙龈上菌斑和牙龈下菌斑），并用于宏基因组文库以进行下一代测序（NGS）制备。
■在特定患者组中的每种临床材料显示出统计学上显著的和数量上不同的细菌组成。I组患者口腔健康状况明显恶化，反映在较高的牙科指数平均值和较高的Veillonella百分比，制革菌,与其他组相比，Capnocytophaga和硒单胞菌属。此外,在所有材料中，两组COVID-19患者的Akkermansia型数量均有统计学意义的下降。
■影响口腔微生物群组成的主要因素不是SARS-CoV-2感染本身，而是使用抗生素治疗。在COVID-19患者中观察到的促炎病原体百分比增加强调了未来预防牙周病和改善口腔卫生的重要性。
