PDF(Pubmed)
Abstract：
Sugar carbonyl groups interact with protein amino groups, forming toxic components referred to as advanced glycation end products (AGEs). The glycation system (BSA, a model protein, and fructose) was incubated for five weeks at 37 °C in the presence and absence of Stevia leaf extract. The results indicated that the leaf extract (0.5 mg/mL) decreased the incidence of browning (70.84 ± 0.08%), fructosamine (67.27 ± 0.08%), and carbonyl content (64.04 ± 0.09%). Moreover, we observed an 81 ± 8.49% reduction in total AGEs. The inhibition of individual AGE (argpyrimidine, vesper lysine, and pentosidine) was ~80%. The decrease in the protein aggregation was observed with Congo red (46.88 ± 0.078%) and the Thioflavin T (31.25 ± 1.18%) methods in the presence of Stevia leaf extract. The repercussion of Stevia leaf extract on DNA glycation was examined using agarose gel electrophoresis, wherein the DNA damage was reversed in the presence of 1 mg/mL of leaf extract. When the HDF cell line was treated with 0.5 mg/mL of extract, the viability of cells decreased by only ~20% along with the same cytokine IL-10 production, and glucose uptake decreased by 28 ± 1.90% compared to the control. In conclusion, Stevia extract emerges as a promising natural agent for mitigating glycation-associated challenges, holding potential for novel therapeutic interventions and enhanced management of its related conditions.
摘要：
糖羰基与蛋白质氨基相互作用，形成称为高级糖基化终产物（AGEs）的有毒成分。糖基化系统（BSA，一种模型蛋白质，和果糖）在存在和不存在甜叶菊叶提取物的情况下在37°C下孵育五周。结果表明，叶提取物（0.5mg/mL）降低了褐变的发生率（70.84±0.08％），果糖胺（67.27±0.08％），和羰基含量(64.04±0.09%)。此外，我们观察到总AGEs减少81±8.49%.抑制个体AGE（嘧啶，vesper赖氨酸，和戊糖苷)为~80%。在存在甜叶菊叶提取物的情况下，刚果红（46.88±0.078％）和硫磺素T（31.25±1.18％）方法观察到蛋白质聚集的减少。使用琼脂糖凝胶电泳检查甜菊叶提取物对DNA糖基化的影响，其中在1mg/mL叶提取物存在下逆转DNA损伤。当HDF细胞系用0.5mg/mL提取物处理时，随着相同的细胞因子IL-10的产生，细胞的活力仅下降了约20％，与对照组相比，葡萄糖摄取减少了28±1.90％。总之,甜叶菊提取物作为缓解糖化相关挑战的一种有前途的天然药物，具有新型治疗干预措施和加强相关疾病管理的潜力。
