Abstract:
The field of plant hormonomics focuses on the qualitative and quantitative analysis of the hormone complement in plant samples, akin to other omics sciences. Plant hormones, alongside primary and secondary metabolites, govern vital processes throughout a plant\'s lifecycle. While active hormones have received significant attention, studying all related compounds provides valuable insights into internal processes. Conventional single-class plant hormone analysis employs thorough sample purification, short analysis and triple quadrupole tandem mass spectrometry. Conversely, comprehensive hormonomics analysis necessitates minimal purification, robust and efficient separation and better-performing mass spectrometry instruments. This review summarizes the current status of plant hormone analysis methods, focusing on sample preparation, advances in chromatographic separation and mass spectrometric detection, including a discussion on internal standard selection and the potential of derivatization. Moreover, current approaches for assessing the spatiotemporal distribution are evaluated. The review touches on the legitimacy of the term plant hormonomics by exploring the current status of methods and outlining possible future trends.
摘要:
植物激素组学领域主要研究植物样品中激素补体的定性和定量分析,类似于其他组学科学。植物激素,除了初级和次级代谢物,管理整个工厂生命周期中的重要过程。虽然活性激素受到了极大的关注,研究所有相关化合物提供了对内部过程的宝贵见解。传统的单类植物激素分析采用彻底的样品纯化,短分析和三重四极杆串联质谱。相反,全面的激素组学分析需要最少的纯化,强大而高效的分离和性能更好的质谱仪器。本文综述了植物激素分析方法的研究现状,专注于样品制备,色谱分离和质谱检测的进展,包括内标选择和衍生化潜力的讨论。此外,评估了当前的时空分布评估方法。该评论通过探索方法的当前状态并概述可能的未来趋势,触及了术语“植物激素组学”的合法性。