Abstract：
CatSper is a voltage dependent calcium ion channel present in the principal piece of sperm tail. It plays a crucial role in sperm hyperactivated motility and so in fertilization. Extracellular loops of mouse sperm CatSper were used to develop a vaccine to achieve protection from pregnancy. These loops were inserted at one of the three hypervariable regions of Human Papilloma Virus (HPV) capsid protein (L1). Recombinant vaccines were expressed in E.coli as inclusion body (IB), purified, refolded and assembled into virus-like particles (VLP) in vitro, and adsorbed on alum. Four vaccine candidates were tested in Balb/C mice. All the constructs proved immunogenic, one showed contraceptive efficacy. This recombinant contraceptive vaccine is a non-hormonal intervention and is expected to give long-acting protection from undesired pregnancies.
摘要：
CatSper是存在于精子尾部主要片段中的电压依赖性钙离子通道。它在精子过度激活运动和受精中起着至关重要的作用。使用小鼠精子CatSper的细胞外环来开发疫苗以实现对妊娠的保护。将这些环插入人乳头瘤病毒(HPV)衣壳蛋白(L1)的三个高变区之一。重组疫苗在大肠杆菌中表达为包涵体（IB），纯化,在体外重新折叠并组装成病毒样颗粒（VLP），吸附在明矾上。在Balb/C小鼠中测试了四种候选疫苗。所有的构建体被证明具有免疫原性，一个显示避孕功效。这种重组避孕疫苗是一种非激素干预措施，有望对不希望的怀孕提供长效保护。
