Abstract：
In recent years, several genome-wide approaches based on RNA sequencing (RNA-seq) have been developed. These methods allow a comprehensive and dynamic view of the structure and function of the multi-layered RNA pathways and networks. Many of these approaches, including the promising one of single-cell transcriptome analysis, have been successfully applied to Pseudomonas aeruginosa. However, we are only at the beginning because only a few surrounding conditions have been considered. Here, we aim to illustrate the different types of approaches based on RNA-seq that will lead us in the future to a better understanding of the dynamics of RNA biology in P. aeruginosa.
摘要：
近年来,已经开发了几种基于RNA测序（RNA-seq）的全基因组方法。这些方法允许对多层RNA途径和网络的结构和功能进行全面和动态的观察。这些方法中的许多，包括有前途的单细胞转录组分析，已成功应用于铜绿假单胞菌。然而,我们只是在开始，因为只考虑了一些周围的条件。这里,我们的目的是说明基于RNA-seq的不同类型的方法，这将使我们在未来更好地理解铜绿假单胞菌RNA生物学的动力学。
